Molecular bases of human diseases
The human body is made of 3.7x1013 cells, which contain about two meters of DNA each. As our cells undergo a total of 1016 divisions in a life time, they synthesize therefore more than 2x1016 meters of DNA, which represents 130,000 times the distance from Earth to the sun! This daunting task is executed by micro-machines called replisomes, containing hundreds of proteins. Replisomes assemble at replication origins and generate DNA structures called replication forks. As they progress along the chromosomes, replisomes often encounter obstacles such as DNA lesions or transcription complexes, leading to replication fork arrest. Stalled forks are fragile structures that can give rise to chromosome breaks and trigger genomic instability if they are not rapidly restarted. Fork stalling occurs even more frequently in cancer cells due to the deregulation of oncogenic pathways. Oncogene-induced replication stress (RS) promotes genomic instability and is therefore the driving force of tumorigenesis. However, RS represents also the Achilles’ heel of cancer cells as it interferes with cell proliferation and sensitizes them to chemotherapeutic agents. Understanding how normal and cancer cells respond to replication stress represents therefore a major challenge in cancer biology.
Our group investigates the cellular responses to replication stress in budding yeast and in human cell lines. Owing to the small size of its genome and the power of molecular genetics, budding yeast is an invaluable model organism to study the RS response and to characterize novel mechanisms that are conserved in human cells and are relevant to cancer biology. This is achieved through the use of powerful technologies to monitor the progression, arrest and recovery of replication forks. These methods include single-molecule approaches such as DNA combing and DNA fiber spreading, chromosome-based assays such as pulsed-field gel electrophoresis and NGS-based assays such as ChIP-seq, BrdU-IP-seq, DRIP-seq and BLESS. Together, these methods provide a comprehensive view of the replication stress response in yeast and human cells, from individual DNA molecules to whole genomes.
To further investigate the links between replication stress and cancer, we have recently teamed up with the group of Jérôme Moreaux (Hematology Department of the University Hospital of Montpellier) who is an expert of the pathophysiology of malignant plasma cells and in particular of Multiple Myeloma (MM).
Hervé Técher, Diyavarshini Gopaul, Jonathan Heuzé , Nail Bouzalmad, Baptiste Leray, Audrey Vernet, Clément Mettling, Jérôme Moreaux, Philippe Pasero, Yea-Lih Lin
Bruyer A, Dutrieux L, de Boussac H, Martin T, Chemlal D, Robert N, Requirand G, Cartron G, Vincent L, Herbaux C, Lutzmann M, Bret C, Pasero P, Moreaux J, Ovejero S
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Epigenome modifications and genomic instability in normal and malignant B cells
Ovejero S, Viziteu E, Dutrieux L, Devin J, Lin YL, Alaterre E, Jourdan M, Basbous J, Requirand G, Robert N, de Boussac H, Seckinger A, Hose D, Vincent L, Herbaux C, Constantinou A, Pasero P, Moreaux J
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Epigenome modifications and genomic instability in normal and malignant B cells
Hurst V, Challa K, Jonas F, Forey R, Sack R, Seebacher J, Schmid CD, Barkai N, Shimada K, Gasser SM, Poli J
Samira Kemiha, Jérôme Poli, Yea-Lih Lin, Armelle Lengronne, Philippe Pasero
Mégane Da Mota, Julien Cau, Julio Mateos-Langerak, Armelle Lengronne, Philippe Pasero, Jérôme Poli
Forey R, Barthe A, Tittel-Elmer M, Wery M, Barrault MB, Ducrot C, Seeber A, Krietenstein N, Szachnowski U, Skrzypczak M, Ginalski K, Rowicka M, Cobb J, Rando OJ, Soutourina J, Dubrana K, Gasser SM, Morillon A, Pasero P, Lengronne A and Poli J
Promonet A, Padioleau I, Liu Y, Sanz L, Biernacka A, Schmitz AL, Skrzypczak M, Sarrazin A, Mettling C, Rowicka M, Ginalski K, Chedin F, Chen CL, Lin YL, Pasero P
Benjamin Pardo, María Moriel‐Carretero, Thibaud Vicat, Andrés Aguilera, Philippe Pasero
Romain Forey, Ana Poveda, Sushma Sharma, Antoine Barthe, Ismael Padioleau, Claire Renard, Robin Lambert, Magdalena Skrzypczak, Krzysztof Ginalski, Armelle Lengronne, Andrei Chabes, Benjamin Pardo, Philippe Pasero
Delamarre A, Barthe A, de la Roche Saint-André C, Luciano P, Forey R, Padioleau I, Skrzypczak M, Ginalski K, Géli V, Pasero P, Lengronne A
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Maintenance of genome integrity during DNA replication
Delamarre A, Barthe A, de la Roche Saint-André C, Luciano P, Forey R, Padioleau I, Skrzypczak M, Ginalski K, Géli V, Pasero P, Lengronne A
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Maintenance of genome integrity during DNA replication
Combes E, Andrade AF, Tosi D, Michaud HA, Coquel F, Garambois V, Desigaud D, Jarlier M, Coquelle A, Pasero P, Bonnefoy N, Moreaux J, Martineau P, Del Rio M, Beijersbergen RL, Vezzio-Vie N, Gongora C
Bianco JN, Bergoglio V, Lin YL, Pillaire MJ, Schmitz AL, Gilhodes J, Lusque A, Mazières J, Lacroix-Triki M, Roumeliotis TI, Choudhary J, Moreaux J, Hoffmann JS, Tourrière H, Pasero P
Herviou L, Jourdan M, Martinez AM, Cavalli G, Moreaux J
Moriel-Carretero M, Pasero P, Pardo B
De Veirman K, Menu E, Maes K, De Beule N, De Smedt E, Maes A, Vlummens P, Fostier K, Kassambara A, Moreaux J, Van Ginderachter JA, De Bruyne E, Vanderkerken K, Van Valckenborgh E
Biernacka A, Zhu Y, Skrzypczak M, Forey R, Pardo B, Grzelak M, Nde J, Mitra A, Kudlicki A, Crosetto N, Pasero P, Rowicka M, Ginalski K
Coquel F, Neumayer C, Lin YL, Pasero P
Herviou L, Kassambara A, Boireau S, Robert N, Requirand G, Müller-Tidow C, Vincent L, Seckinger A, Goldschmidt H, Cartron G, Hose D, Cavalli G, Moreaux J
Bacal J, Moriel-Carretero M, Pardo B, Barthe A, Sharma S, Chabes A, Lengronne A, Pasero P
Sasi NK, Coquel F, Lin YL, MacKeigan JP, Pasero P, Weinreich M
Ashi MO, Srour N, Lambert JM, Marchalot A, Martin O, Le Noir S, Pinaud E, Ayala MV, Sirac C, Saulière J, Moreaux J, Cogné M, Delpy L
Herviou L, Cavalli G, Moreaux J
Coquel F, Silva MJ, Técher H, Zadorozhny K, Sharma S, Nieminuszczy J, Mettling C, Dardillac E, Barthe A, Schmitz AL, Promonet A, Cribier A, Sarrazin A, Niedzwiedz W, Lopez B, Costanzo V, Krejci L, Chabes A, Benkirane M, Lin YL, Pasero P
Bruyer A, Maes K, Herviou L, Kassambara A, Seckinger A, Cartron G, Rème T, Robert N, Requirand G, Boireau S, Müller-Tidow C, Veyrune JL, Vincent L, Bouhya S, Goldschmidt H, Vanderkerken K, Hose D, Klein B, De Bruyne E, Moreaux J
Cohen S, Puget N, Lin YL, Clouaire T, Aguirrebengoa M, Rocher V, Pasero P, Canitrot Y, Legube G
Fang D, Lengronne A, Shi D, Forey R, Skrzypczak M, Ginalski K, Yan C, Wang X, Cao Q, Pasero P, Lou H
De Smedt E, Maes K, Verhulst S, Lui H, Kassambara A, Maes A, Robert N, Heirman C, Cakana A, Hose D, Breckpot K, van Grunsven LA, De Veirman K, Menu E, Vanderkerken K, Moreaux J, De Bruyne E
Alaterre E, Raimbault S, Goldschmidt H, Bouhya S, Requirand G, Robert N, Boireau S, Seckinger A, Hose D, Klein B, Moreaux J
De Veirman K, De Beule N, Maes K, Menu E, De Bruyne E, De Raeve H, Fostier K, Moreaux J, Kassambara A, Hose D, Heusschen R, Eriksson H, Vanderkerken K, Van Valckenborgh E.
Alaterre E, Raimbault S, Garcia JM, Rème T, Requirand G, Klein B, Moreaux J.
Lin, YL., Pasero, P.
Jourdan M, Robert N, Cren M, Thibaut C, Duperray C, Kassambara A, Cogné M, Tarte K, Klein B, Moreaux J.
Schoenhals M, Jourdan M, Bruyer A, Kassambara A, Klein B, Moreaux J.
Kassambara A, Jourdan M, Bruyer A, Robert N, Pantesco V, Elemento O, Klein B, Moreaux J.
Viziteu E, Klein B, Basbous J, Lin YL, Hirtz C, Gourzones C, Tiers L, Bruyer A, Vincent L, Grandmougin C, Seckinger A, Goldschmidt H, Constantinou A, Pasero P, Hose D, Moreaux J.
Uzcanga G, Lara E, Gutiérrez F, Beaty D, Beske T, Teran R, Navarro JC, Pasero P, Benítez W, Poveda A
Pardo B, Crabbé L, Pasero P
Pasero P, Vindigni A.
Herviou L, Cavalli G, Cartron G, Klein B, Moreaux J.
Seeber A, Hegnauer AM, Hustedt N, Deshpande I, Poli J, Eglinger J, Pasero P, Gut H, Shinohara M, Hopfner KP, Shimada K, Gasser SM.
Bastia D, Srivastava P, Zaman S, Choudhury M, Mohanty BK, Bacal J, Langston LD, Pasero P, O'Donnell ME
Schoenhals M, Jourdan M, Seckinger A, Pantesco V, Hose D, Kassambara A, Moreaux J, Klein B.
Ogrunc M, Martinez-Zamudio RI, Sadoun PB, Dore G, Schwerer H, Pasero P, Lemaitre JM, Dejean A, Bischof O
Jourdan M, Cren M, Schafer P, Robert N, Duperray C, Vincent L, Ceballos P, Cartron G, Rossi JF, Moreaux J, Chopra R, Klein B
Viziteu E, Grandmougin C, Goldschmidt H, Seckinger A, Hose D, Klein B, Moreaux J.
Viziteu E, Kassambara A, Pasero P, Klein B, Moreaux J.
Poli J, Gerhold CB, Tosi A, Hustedt N, Seeber A, Sack R, Herzog F, Pasero P, Shimada K, Hopfner KP, Gasser SM.
Lub S, Maes A, Maes K, De Veirman K, De Bruyne E, Menu E, Fostier K, Kassambara A, Moreaux J, Hose D, Leleu X, King RW, Vanderkerken K, Van Valckenborgh E.
Bret C, Viziteu E, Kassambara A, Moreaux J.
Bret C, Klein B, Cartron G, Schved JF, Constantinou A, Pasero P, Moreaux J.
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Genetic Instability and Cancer
Menolfi D, Delamarre A, Lengronne A, Pasero P, Branzei D.
Seckinger A, Meißner T, Moreaux J, Benes V, Hillengass J, Castoldi M, Zimmermann J, Ho AD, Jauch A, Goldschmidt H, Klein B, Hose D.
Lapierre M, Castet-Nicolas A, Gitenay D, Jalaguier S, Teyssier C, Bret C, Cartron G, Moreaux J, Cavaillès V. J Hematol Oncol. 2015 Mar 4;8(1):20.
Maes K, De Smedt E, Kassambara A, Hose D, Seckinger A, Van Valckenborgh E, Menu E, Klein B, Vanderkerken K, Moreaux J, De Bruyne E
Hassen W, Kassambara A, Reme T, Sahota S, Seckinger A, Vincent L, Cartron G, Moreaux J, Hose D, Klein B
Kassambara A, Gourzones-Dmitriev C, Sahota S, Rème T, Moreaux J, Goldschmidt H, Constantinou A, Pasero P, Hose D, Klein B
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Genetic Instability and Cancer
Clifford R, Louis T, Robbe P, Ackroyd S, Burns A, Timbs AT, Colopy GW, Dreau H, Sigaux F, Judde JG, Rotger M, Telenti A, Lin YL, Pasero P, Maelfait J, Titsias M, Cohen DR, Henderson SJ, Ross M, Bentley D, Hillmen P, Pettitt A, Rehwinkel J, Knight SJ, Taylor JC, Crow YJ, Benkirane M, Schuh A.
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Laboratory of Molecular Virology
Lin YL, Pasero P.
Lengronne A, Pasero P.
Yoshida K, Bacal J, Desmarais D, Padioleau I, Tsaponina O, Chabes A, Pantesco V, Dubois E, Parrinello H, Skrzypczak M, Ginalski K, Lengronne A, Pasero P
Magdalou I, Lopez BS, Pasero P, Lambert SA
Sheu YJ, Kinney JB, Lengronne A, Pasero P, Stillman B.
Mejlvang J, Feng Y, Alabert C, Neelsen KJ, Jasencakova Z, Zhao X, Lees M, Sandelin A, Pasero P, Lopes M, Groth A.
Méchali M, Yoshida K, Coulombe P, Pasero P.
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Replication and Genome Dynamics
Sokol AM, Cruet-Hennequart S, Pasero P, Carty MP.
Yoshida K, Poveda A, Pasero P.
Gourzones-Dmitriev C, Kassambara A, Sahota S, Rème T, Moreaux J, Bourquard P, Hose D, Pasero P, Constantinou A, Klein B.
Yeeles JT, Poli J, Marians KJ, Pasero P.
Crosetto N, Mitra A, Silva MJ, Bienko M, Dojer N, Wang Q, Karaca E, Chiarle R, Skrzypczak M, Ginalski K, Pasero P, Rowicka M, Dikic I
Lin YL, Pasero P.
Tittel-Elmer M, Lengronne A, Davidson MB, Bacal J, François P, Hohl M, Petrini JH, Pasero P, Cobb JA.
Bianco JN, Poli J, Saksouk J, Bacal J, Silva MJ, Yoshida K, Lin YL, Tourrière H, Lengronne A, Pasero P.
Poli J, Tsaponina O, Crabbé L, Keszthelyi A, Pantesco V, Chabes A, Lengronne A, Pasero P.
Wurtele H, Kaiser GS, Bacal J, St-Hilaire E, Lee EH, Tsao S, Dorn J, Maddox P, Lisby M, Pasero P, Verreault A.
Allera-Moreau C, Rouquette I, Lepage B, Oumouhou N, Walschaerts M, Leconte E, Schilling V, Gordien K, Brouchet L, Delisle MB, Mazieres J, Hoffmann JS, Pasero P, Cazaux C.
Gongora, C., Vezzio-Vie, N., Tuduri, S., Denis, V., Causse, A., Auzanneau, C., Collod-Beroud, G., Coquelle, A., Pasero, P., Pourquier, P., Martineau, P., Del Rio, M
Pillaire, M.J., Selves, J., Gordien, K., Gouraud, P.A., Gentil, C., Danjoux, M., Do, C., Negre, V., Bieth, A., Guimbaud, R., Trouche, D., Pasero, P., Méchali, M., Hoffmann, JS, and Cazaux, C
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Replication and Genome Dynamics
Poveda, AM., Le Clech, M., Pasero, P.
Piwko, W., Olma, MH., Held, M., Bianco, JN., Pedrioli, PG., Hofmann, K., Pasero, P., Gerlich, DW., Peter, M
Tuduri, S., Crabbé, L., Conti, C., Tourrière, H., Holtgreve-Grez, H., Jauch, A., Pantesco, V., De Vos, J., Thomas, A., Theillet, C., Pommier, Y., Tazi, J., Coquelle, A., Pasero, P
Crabbe, L., Thomas, A., Pantesco, V., De Vos, J., Pasero, P. and Lengronne, A.
Giannattasio M, Follonier C, Tourrière H, Puddu F, Lazzaro F, Pasero P, Lopes M, Plevani P, Muzi-Falconi M
Bermúdez-López, M., Ceschia, A., de Piccoli, G., Colomina, N., Pasero, P., Aragón, L., Torres-Rosell, J.
Tuduri, S., Tourrière, H., Pasero, P.
Tuduri, S., Crabbe, L., Tourrière, H., Coquelle, A., Pasero, P.
Tuduri, S., Tourrière, H., Pasero, P
Falbo, K.B., Alabert, C., Katou, Y., Wu, S., Han, J., Wehr, T., Xiao, J., He, X., Zhang, Z., Shi, Y., Shirahige, K., Pasero, P., Shen, X.
Tuduri S, Crabbé L, Conti C, Tourrière H, Holtgreve-Grez H, Jauch A, Pantesco V, De Vos J, Thomas A, Theillet C, Pommier Y, Tazi J, Coquelle A, Pasero P.
Alabert, C., Poveda, A. and Pasero, P
Marqués, M., Kumar, A., Poveda, AM., Zuluaga, S., Hernández, C., Jackson, S., Pasero, P., Carrera, AC.
Tittel-Elmer M, Alabert C, Pasero P, Cobb JA.
Alabert C, Bianco JN, Pasero P
Zaidi, IW., Rabut, G., Poveda, A., Scheel, H., Malmström, J., Ulrich, H., Hofmann, K., Pasero, P., Peter, M., Luke, B.
Solovei, I., Pasero, P., Visa, N.
Flott, S., Alabert, C., Toh, GW., Toth, R., Sugawara, N., Campbell, DG., Haber, JE., Pasero, P., Rouse, J.
Pillaire MJ, Betous R, Conti C, Czaplicki J, Pasero P, Bensimon A, Cazaux C, Hoffmann JS.
Torres-Rosell J, De Piccoli G, Cordon-Preciado V, Farmer S, Jarmuz A, Machin F, Pasero P, Lisby M, Haber JE, Aragon L.
Tourriere, H., Pasero Ph.
Semple JW, Da-Silva LF, Jervis EJ, Ah-Kee J, Al-Attar H, Kummer L, Heikkila JJ, Pasero P, Duncker BP.
Luke, B, Versini, G, Jaquenoud, M, Zaidi, IW, Kurz, T, Pintard, L, Pasero, P, Peter, M.
Tourriere, H., Versini, G., Cordon-Preciado, V., Alabert, C., Pasero, P.
Defended by Samira Kemiha on 14/04/2022 under the supervision of Armelle Lengronne and Jérôme Poli
How do cells coordinate replication and transcription?
By Jérôme Poli on 08/04/2022
Defended by Romain Forey on 15/11/2019 under the supervision of Philippe Pasero
Defended by Flavie Coquel on 19/09/2018 under the supervision of Yea-Lih Lin and Philippe Pasero
Defended by Ismaël Padioleau on 24/11/2017 under the supervision of Philippe Pasero
Defended by Alexy Promonet on 15/12/2016 under the supervision of Yea-Lih Lin and Philippe Pasero
