Maintenance of genome integrity during DNA replication

Molecular bases of human diseases

The human body is made of 3.7x1013 cells, which contain about two meters of DNA each. As our cells undergo a total of 1016 divisions in a life time, they synthesize therefore more than 2x1016 meters of DNA, which represents 130,000 times the distance from Earth to the sun! This daunting task is executed by micro-machines called replisomes, containing hundreds of proteins. Replisomes assemble at replication origins and generate DNA structures called replication forks. As they progress along the chromosomes, replisomes often encounter obstacles such as DNA lesions or transcription complexes, leading to replication fork arrest. Stalled forks are fragile structures that can give rise to chromosome breaks and trigger genomic instability if they are not rapidly restarted. Fork stalling occurs even more frequently in cancer cells due to the deregulation of oncogenic pathways. Oncogene-induced replication stress (RS) promotes genomic instability and is therefore the driving force of tumorigenesis. However, RS represents also the Achilles’ heel of cancer cells as it interferes with cell proliferation and sensitizes them to chemotherapeutic agents. Understanding how normal and cancer cells respond to replication stress represents therefore a major challenge in cancer biology.

Our group investigates the cellular responses to replication stress in budding yeast and in human cell lines. Owing to the small size of its genome and the power of molecular genetics, budding yeast is an invaluable model organism to study the RS response and to characterize novel mechanisms that are conserved in human cells and are relevant to cancer biology. This is achieved through the use of powerful technologies to monitor the progression, arrest and recovery of replication forks. These methods include single-molecule approaches such as DNA combing and DNA fiber spreading, chromosome-based assays such as pulsed-field gel electrophoresis and NGS-based assays such as ChIP-seq, BrdU-IP-seq, DRIP-seq and BLESS. Together, these methods provide a comprehensive view of the replication stress response in yeast and human cells, from individual DNA molecules to whole genomes.

To further investigate the links between replication stress and cancer, we have recently teamed up with the group of Jérôme Moreaux (Hematology Department of the University Hospital of Montpellier) who is an expert of the pathophysiology of malignant plasma cells and in particular of Multiple Myeloma (MM).

 

Bayan Chami
Bayan CHAMI
Researcher

0434359943
Yea-Lih LIN
Researcher

0434359943
Benjamin PARDO
Researcher

0434359943
Philippe PASERO
Director
Researcher

0434359929
Jérôme POLI
Researcher

0434359955
Laura Chaptal
Laura CHAPTAL
Doctorante
PhD Student

0434359955
Mathilde Courtes
Mathilde COURTES
PhD Student

0434359955
Mégane DA MOTA
PhD Student

0434359955
Jonathan HEUZE
PhD Student

0434359951
Silpa Mary Johnson
Silpa Mary JOHNSON
PhD Student

0434359943
Silpa Mary Johnson
Silpa Mary JOHNSON
PhD Student

0434359943
Chun-Yen Yang
Chun-Yen YANG
PhD Student

0434359943
Antoine BARTHE
ITA

0434359955
Nail Bouzalmad
Nail BOUZALMAD
ITA

0434359943
Giovanni Nigro
Giovanni NIGRO
Stagiaire

0411759728

Publications of the team

RNase H2 degrades toxic RNA:DNA hybrids behind stalled forks to promote replication restart

The BLM helicase is a new therapeutic target in multiple myeloma involved in replication stress survival and drug resistance.

Ovejero S, Viziteu E, Dutrieux L, Devin J, Lin YL, Alaterre E, Jourdan M, Basbous J, Requirand G, Robert N, de Boussac H, Seckinger A, Hose D, Vincent L, Herbaux C, Constantinou A, Pasero P, Moreaux J


+ Epigenome modifications and genomic instability in normal and malignant B cells

+ Genetic Instability and Cancer

more

A regulatory phosphorylation site on Mec1 controls chromatin occupancy of RNA polymerases during replication stress.

Hurst V, Challa K, Jonas F, Forey R, Sack R, Seebacher J, Schmid CD, Barkai N, Shimada K, Gasser SM, Poli J

Replication stress: from chromatin to immunity and beyond.

Lin YL, Pasero P

read publication

Toxic R-loops: Cause or consequence of replication stress?

Samira Kemiha, Jérôme Poli, Yea-Lih Lin, Armelle Lengronne, Philippe Pasero

more

3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei

Mégane Da Mota, Julien Cau, Julio Mateos-Langerak, Armelle Lengronne, Philippe Pasero, Jérôme Poli

A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization

Forey R, Barthe A, Tittel-Elmer M, Wery M, Barrault MB, Ducrot C, Seeber A, Krietenstein N, Szachnowski U, Skrzypczak M, Ginalski K, Rowicka M, Cobb J, Rando OJ, Soutourina J, Dubrana K, Gasser SM, Morillon A, Pasero P, Lengronne A and Poli J

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Topoisomerase 1 prevents replication stress at R-loop-enriched transcription termination sites

Promonet A, Padioleau I, Liu Y, Sanz L, Biernacka A, Schmitz AL, Skrzypczak M, Sarrazin A, Mettling C, Rowicka M, Ginalski K, Chedin F, Chen CL, Lin YL, Pasero P

more

Homologous recombination and Mus81 promote replication completion in response to replication fork blockage

Benjamin Pardo, María Moriel‐Carretero, Thibaud Vicat, Andrés Aguilera, Philippe Pasero

more

Mec1 Is Activated at the Onset of Normal S Phase by Low-dNTP Pools Impeding DNA Replication

Romain Forey, Ana Poveda, Sushma Sharma, Antoine Barthe, Ismael Padioleau, Claire Renard, Robin Lambert, Magdalena Skrzypczak, Krzysztof Ginalski, Armelle Lengronne, Andrei Chabes, Benjamin Pardo, Philippe Pasero

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MRX Increases Chromatin Accessibility at Stalled Replication Forks to Promote Nascent DNA Resection and Cohesin Loading.

Delamarre A, Barthe A, de la Roche Saint-André C, Luciano P, Forey R, Padioleau I, Skrzypczak M, Ginalski K, Géli V, Pasero P, Lengronne A


+ Maintenance of genome integrity during DNA replication

MRX Increases Chromatin Accessibility at Stalled Replication Forks to Promote Nascent DNA Resection and Cohesin Loading.

Delamarre A, Barthe A, de la Roche Saint-André C, Luciano P, Forey R, Padioleau I, Skrzypczak M, Ginalski K, Géli V, Pasero P, Lengronne A


+ Maintenance of genome integrity during DNA replication

Inhibition of Ataxia-Telangiectasia Mutated and RAD3-related (ATR) overcomes oxaliplatin resistance and promotes anti-tumor immunity in colorectal cancer.

Combes E, Andrade AF, Tosi D, Michaud HA, Coquel F, Garambois V, Desigaud D, Jarlier M, Coquelle A, Pasero P, Bonnefoy N, Moreaux J, Martineau P, Del Rio M, Beijersbergen RL, Vezzio-Vie N, Gongora C

Overexpression of Claspin and Timeless protects cancer cells from replication stress in a checkpoint-independent manner.

Bianco JN, Bergoglio V, Lin YL, Pillaire MJ, Schmitz AL, Gilhodes J, Lusque A, Mazières J, Lacroix-Triki M, Roumeliotis TI, Choudhary J, Moreaux J, Hoffmann JS, Tourrière H, Pasero P

EZH2 is overexpressed in transitional preplasmablasts and is involved in human plasma cell differentiation.

Herviou L, Jourdan M, Martinez AM, Cavalli G, Moreaux J

+ Chromatin and cell biology

DDR Inc., one business, two associates.

Moriel-Carretero M, Pasero P, Pardo B

Myeloid-derived suppressor cells induce multiple myeloma cell survival by activating the AMPK pathway.

De Veirman K, Menu E, Maes K, De Beule N, De Smedt E, Maes A, Vlummens P, Fostier K, Kassambara A, Moreaux J, Van Ginderachter JA, De Bruyne E, Vanderkerken K, Van Valckenborgh E

i-BLESS is an ultra-sensitive method for detection of DNA double-strand breaks.

Biernacka A, Zhu Y, Skrzypczak M, Forey R, Pardo B, Grzelak M, Nde J, Mitra A, Kudlicki A, Crosetto N, Pasero P, Rowicka M, Ginalski K

SAMHD1 and the innate immune response to cytosolic DNA during DNA replication.

Coquel F, Neumayer C, Lin YL, Pasero P

PRC2 targeting is a therapeutic strategy for EZ score defined high-risk multiple myeloma patients and overcome resistance to IMiDs.

Herviou L, Kassambara A, Boireau S, Robert N, Requirand G, Müller-Tidow C, Vincent L, Seckinger A, Goldschmidt H, Cartron G, Hose D, Cavalli G, Moreaux J

+ Chromatin and cell biology

Mrc1 and Rad9 cooperate to regulate initiation and elongation of DNA replication in response to DNA damage.

Bacal J, Moriel-Carretero M, Pardo B, Barthe A, Sharma S, Chabes A, Lengronne A, Pasero P

DDK Has a Primary Role in Processing Stalled Replication Forks to Initiate Downstream Checkpoint Signaling.

Sasi NK, Coquel F, Lin YL, MacKeigan JP, Pasero P, Weinreich M

Physiological and druggable skipping of immunoglobulin variable exons in plasma cells.

Ashi MO, Srour N, Lambert JM, Marchalot A, Martin O, Le Noir S, Pinaud E, Ayala MV, Sirac C, Saulière J, Moreaux J, Cogné M, Delpy L

[EZH2 is therapeutic target for personalized treatment in multiple myeloma].

Herviou L, Cavalli G, Moreaux J

+ Chromatin and cell biology

SAMHD1 acts at stalled replication forks to prevent interferon induction.

Coquel F, Silva MJ, Técher H, Zadorozhny K, Sharma S, Nieminuszczy J, Mettling C, Dardillac E, Barthe A, Schmitz AL, Promonet A, Cribier A, Sarrazin A, Niedzwiedz W, Lopez B, Costanzo V, Krejci L, Chabes A, Benkirane M, Lin YL, Pasero P

DNMTi/HDACi combined epigenetic targeted treatment induces reprogramming of myeloma cells in the direction of normal plasma cells.

Bruyer A, Maes K, Herviou L, Kassambara A, Seckinger A, Cartron G, Rème T, Robert N, Requirand G, Boireau S, Müller-Tidow C, Veyrune JL, Vincent L, Bouhya S, Goldschmidt H, Vanderkerken K, Hose D, Klein B, De Bruyne E, Moreaux J

Senataxin resolves RNA:DNA hybrids forming at DNA double-strand breaks to prevent translocations.

Cohen S, Puget N, Lin YL, Clouaire T, Aguirrebengoa M, Rocher V, Pasero P, Canitrot Y, Legube G

Dbf4 recruitment by forkhead transcription factors defines an upstream rate-limiting step in determining origin firing timing.

Fang D, Lengronne A, Shi D, Forey R, Skrzypczak M, Ginalski K, Yan C, Wang X, Cao Q, Pasero P, Lou H

Loss of RASSF4 Expression in Multiple Myeloma Promotes RAS-Driven Malignant Progression.

De Smedt E, Maes K, Verhulst S, Lui H, Kassambara A, Maes A, Robert N, Heirman C, Cakana A, Hose D, Breckpot K, van Grunsven LA, De Veirman K, Menu E, Vanderkerken K, Moreaux J, De Bruyne E

CD24, CD27, CD36 and CD302 gene expression for outcome prediction in patients with multiple myeloma.

Alaterre E, Raimbault S, Goldschmidt H, Bouhya S, Requirand G, Robert N, Boireau S, Seckinger A, Hose D, Klein B, Moreaux J

Extracellular S100A9 Protein in Bone Marrow Supports Multiple Myeloma Survival by Stimulating Angiogenesis and Cytokine Secretion.

De Veirman K, De Beule N, Maes K, Menu E, De Bruyne E, De Raeve H, Fostier K, Moreaux J, Kassambara A, Hose D, Heusschen R, Eriksson H, Vanderkerken K, Van Valckenborgh E.

Automated and simplified identification of normal and abnormal plasma cells in Multiple Myeloma by flow cytometry

Alaterre E, Raimbault S, Garcia JM, Rème T, Requirand G, Klein B, Moreaux J.

Transcription-Replication Conflicts: Orientation Matters

Lin, YL., Pasero, P.

Characterization of human FCRL4-positive B cells

Jourdan M, Robert N, Cren M, Thibaut C, Duperray C, Kassambara A, Cogné M, Tarte K, Klein B, Moreaux J.

Hypoxia favors the generation of human plasma cells

Schoenhals M, Jourdan M, Bruyer A, Kassambara A, Klein B, Moreaux J.

Global miRNA expression analysis identifies novel key regulators of plasma cell differentiation and malignant plasma cell.

Kassambara A, Jourdan M, Bruyer A, Robert N, Pantesco V, Elemento O, Klein B, Moreaux J.

RECQ1 helicase is involved in replication stress survival and drug resistance in multiple myeloma

Viziteu E, Klein B, Basbous J, Lin YL, Hirtz C, Gourzones C, Tiers L, Bruyer A, Vincent L, Grandmougin C, Seckinger A, Goldschmidt H, Constantinou A, Pasero P, Hose D, Moreaux J.

+ Genetic Instability and Cancer

Nuclear DNA replication and repair in parasites of the genus Leishmania: Exploiting differences to develop innovative therapeutic approaches

Uzcanga G, Lara E, Gutiérrez F, Beaty D, Beske T, Teran R, Navarro JC, Pasero P, Benítez W, Poveda A

Signaling Pathways of Replication Stress in Yeast

Pardo B, Crabbé L, Pasero P

Nucleases Acting at Stalled Forks: How to Reboot the Replication Program with a Few Shortcuts

Pasero P, Vindigni A.

EZH2 in normal hematopoiesis and hematological malignancies

Herviou L, Cavalli G, Cartron G, Klein B, Moreaux J.


+ Chromatin and cell biology

RPA Mediates Recruitment of MRX to Forks and Double-Strand Breaks to Hold Sister Chromatids Together

Seeber A, Hegnauer AM, Hustedt N, Deshpande I, Poli J, Eglinger J, Pasero P, Gut H, Shinohara M, Hopfner KP, Shimada K, Gasser SM.

Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest

Bastia D, Srivastava P, Zaman S, Choudhury M, Mohanty BK, Bacal J, Langston LD, Pasero P, O'Donnell ME

Forced KLF4 expression increases the generation of mature plasma cells and uncovers a network linked with plasma cell stage

Schoenhals M, Jourdan M, Seckinger A, Pantesco V, Hose D, Kassambara A, Moreaux J, Klein B.

USP1 Regulates Cellular Senescence by Controlling Genomic Integrity

Ogrunc M, Martinez-Zamudio RI, Sadoun PB, Dore G, Schwerer H, Pasero P, Lemaitre JM, Dejean A, Bischof O

Differential effects of lenalidomide during plasma cell differentiation

Jourdan M, Cren M, Schafer P, Robert N, Duperray C, Vincent L, Ceballos P, Cartron G, Rossi JF, Moreaux J, Chopra R, Klein B

Chetomin, targeting HIF-1α/p300 complex, exhibits antitumour activity in multiple myeloma

Viziteu E, Grandmougin C, Goldschmidt H, Seckinger A, Hose D, Klein B, Moreaux J.

RECQ helicases are deregulated in hematological malignancies in association with a prognostic value

Viziteu E, Kassambara A, Pasero P, Klein B, Moreaux J.

Mec1, INO80, and the PAF1 complex cooperate to limit transcription replication conflicts through RNAPII removal during replication stress

Poli J, Gerhold CB, Tosi A, Hustedt N, Seeber A, Sack R, Herzog F, Pasero P, Shimada K, Hopfner KP, Gasser SM.

Inhibiting the anaphase promoting complex/cyclosome induces a metaphase arrest and cell death in multiple myeloma cells

Lub S, Maes A, Maes K, De Veirman K, De Bruyne E, Menu E, Fostier K, Kassambara A, Moreaux J, Hose D, Leleu X, King RW, Vanderkerken K, Van Valckenborgh E.

Identifying high-risk adult AML patients: epigenetic and genetic risk factors and their implications for therapy

Bret C, Viziteu E, Kassambara A, Moreaux J.

DNA repair in diffuse large B-cell lymphoma: a molecular portrait

Bret C, Klein B, Cartron G, Schved JF, Constantinou A, Pasero P, Moreaux J.


+ Genetic Instability and Cancer

Essential Roles of the Smc5/6 Complex in Replication through Natural Pausing Sites and Endogenous DNA Damage Tolerance

Menolfi D, Delamarre A, Lengronne A, Pasero P, Branzei D.

miRNAs in multiple myeloma - a survival relevant complex regulator of gene expression

Seckinger A, Meißner T, Moreaux J, Benes V, Hillengass J, Castoldi M, Zimmermann J, Ho AD, Jauch A, Goldschmidt H, Klein B, Hose D.

Expression and role of RIP140/NRIP1 in chronic lymphocytic leukemia

Lapierre M, Castet-Nicolas A, Gitenay D, Jalaguier S, Teyssier C, Bret C, Cartron G, Moreaux J, Cavaillès V. J Hematol Oncol. 2015 Mar 4;8(1):20.

In vivo treatment with epigenetic modulating agents induces transcriptional alterations associated with prognosis and immunomodulation in multiple myeloma

Maes K, De Smedt E, Kassambara A, Hose D, Seckinger A, Van Valckenborgh E, Menu E, Klein B, Vanderkerken K, Moreaux J, De Bruyne E

Drug metabolism and clearance system in tumor cells of patients with multiple myeloma

Hassen W, Kassambara A, Reme T, Sahota S, Seckinger A, Vincent L, Cartron G, Moreaux J, Hose D, Klein B

A DNA repair pathway score predicts survival in human multiple myeloma: the potential for therapeutic strategy

Kassambara A, Gourzones-Dmitriev C, Sahota S, Rème T, Moreaux J, Goldschmidt H, Constantinou A, Pasero P, Hose D, Klein B


+ Genetic Instability and Cancer

SAMHD1 is mutated recurrently in chronic lymphocytic leukemia and is involved in response to DNA damage

Clifford R, Louis T, Robbe P, Ackroyd S, Burns A, Timbs AT, Colopy GW, Dreau H, Sigaux F, Judde JG, Rotger M, Telenti A, Lin YL, Pasero P, Maelfait J, Titsias M, Cohen DR, Henderson SJ, Ross M, Bentley D, Hillmen P, Pettitt A, Rehwinkel J, Knight SJ, Taylor JC, Crow YJ, Benkirane M, Schuh A.


+ Laboratory of Molecular Virology

Caught in the Act: R-Loops Are Cleaved by Structure-Specific Endonucleases to Generate DSBs

Lin YL, Pasero P.

Closing the MCM cycle at replication termination sites

Lengronne A, Pasero P.

The histone deacetylases sir2 and rpd3 act on ribosomal DNA to control the replication program in budding yeast.

Yoshida K, Bacal J, Desmarais D, Padioleau I, Tsaponina O, Chabes A, Pantesco V, Dubois E, Parrinello H, Skrzypczak M, Ginalski K, Lengronne A, Pasero P

The causes of replication stress and their consequences on genome stability and cell fate

Magdalou I, Lopez BS, Pasero P, Lambert SA

Domain within the helicase subunit Mcm4 integrates multiple kinase signals to control DNA replication initiation and fork progression

Sheu YJ, Kinney JB, Lengronne A, Pasero P, Stillman B.

New histone supply regulates replication fork speed and PCNA unloading

Mejlvang J, Feng Y, Alabert C, Neelsen KJ, Jasencakova Z, Zhao X, Lees M, Sandelin A, Pasero P, Lopes M, Groth A.

Genetic and epigenetic determinants of DNA replication origins, position and activation

Méchali M, Yoshida K, Coulombe P, Pasero P.


+ Replication and Genome Dynamics

DNA polymerase η modulates replication fork progression and DNA damage responses in platinum-treated human cells

Sokol AM, Cruet-Hennequart S, Pasero P, Carty MP.

Time to Be Versatile: Regulation of the Replication Timing Program in Budding Yeast

Yoshida K, Poveda A, Pasero P.

DNA repair pathways in human multiple myeloma: Role in oncogenesis and potential targets for treatment.

Gourzones-Dmitriev C, Kassambara A, Sahota S, Rème T, Moreaux J, Bourquard P, Hose D, Pasero P, Constantinou A, Klein B.

+ Genetic Instability and Cancer

Rescuing stalled or damaged replication forks

Yeeles JT, Poli J, Marians KJ, Pasero P.

Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing

Crosetto N, Mitra A, Silva MJ, Bienko M, Dojer N, Wang Q, Karaca E, Chiarle R, Skrzypczak M, Ginalski K, Pasero P, Rowicka M, Dikic I

Interference between DNA replication and transcription as a cause of genomic instability

Lin YL, Pasero P.

Cohesin Association to Replication Sites Depends on Rad50 and Promotes Fork Restart.

Tittel-Elmer M, Lengronne A, Davidson MB, Bacal J, François P, Hohl M, Petrini JH, Pasero P, Cobb JA.

Analysis of DNA replication profiles in budding yeast and mammalian cells using DNA combing

Bianco JN, Poli J, Saksouk J, Bacal J, Silva MJ, Yoshida K, Lin YL, Tourrière H, Lengronne A, Pasero P.

dNTP pools determine fork progression and origin usage under replication stress.

Poli J, Tsaponina O, Crabbé L, Keszthelyi A, Pantesco V, Chabes A, Lengronne A, Pasero P.

Histone h3 lysine 56 acetylation and the response to DNA replication fork damage.

Wurtele H, Kaiser GS, Bacal J, St-Hilaire E, Lee EH, Tsao S, Dorn J, Maddox P, Lisby M, Pasero P, Verreault A.

DNA replication stress response involving PLK1, CDC6, POLQ, RAD51 and CLASPIN upregulation prognoses the outcome of early/mid-stage non-small cell lung cancer patients

Allera-Moreau C, Rouquette I, Lepage B, Oumouhou N, Walschaerts M, Leconte E, Schilling V, Gordien K, Brouchet L, Delisle MB, Mazieres J, Hoffmann JS, Pasero P, Cazaux C.

New Topoisomerase I mutations are associated with resistance to camptothecin

Gongora, C., Vezzio-Vie, N., Tuduri, S., Denis, V., Causse, A., Auzanneau, C., Collod-Beroud, G., Coquelle, A., Pasero, P., Pourquier, P., Martineau, P., Del Rio, M

A DNA replication signature of progression and negative outcome in colorectal cancer

Pillaire, M.J., Selves, J., Gordien, K., Gouraud, P.A., Gentil, C., Danjoux, M., Do, C., Negre, V., Bieth, A., Guimbaud, R., Trouche, D., Pasero, P., Méchali, M., Hoffmann, JS, and Cazaux, C


+ Replication and Genome Dynamics

Transcription and replication: Breaking the rules of the road causes genomic instability

Poveda, AM., Le Clech, M., Pasero, P.

RNAi-based screening identifies the Mms22L-Nfkbil2 complex as a novel regulator of DNA replication in human cells

Piwko, W., Olma, MH., Held, M., Bianco, JN., Pedrioli, PG., Hofmann, K., Pasero, P., Gerlich, DW., Peter, M

Topoisomerase I suppresses genomic instability by preventing interference between replication and transcription

Tuduri, S., Crabbé, L., Conti, C., Tourrière, H., Holtgreve-Grez, H., Jauch, A., Pantesco, V., De Vos, J., Thomas, A., Theillet, C., Pommier, Y., Tazi, J., Coquelle, A., Pasero, P

Analysis of replication profiles reveals key role of RFC-Ctf18 in yeast replication stress response.

Crabbe, L., Thomas, A., Pantesco, V., De Vos, J., Pasero, P. and Lengronne, A.

Exo1 competes with repair synthesis, converts NER intermediates to long ssDNA gaps, and promotes checkpoint activation.

Giannattasio M, Follonier C, Tourrière H, Puddu F, Lazzaro F, Pasero P, Lopes M, Plevani P, Muzi-Falconi M

The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages

Bermúdez-López, M., Ceschia, A., de Piccoli, G., Colomina, N., Pasero, P., Aragón, L., Torres-Rosell, J.

Defining replication origin efficiency using DNA fiber assays.

Tuduri, S., Tourrière, H., Pasero, P.

Does interference between replication and transcription contribute to genomic instability in cancer cells?

Tuduri, S., Crabbe, L., Tourrière, H., Coquelle, A., Pasero, P.

Defining replication origin efficiency using DNA fiber assays

Tuduri, S., Tourrière, H., Pasero, P

Involvement of a chromatin remodeling complex in damage tolerance during DNA replication

Falbo, K.B., Alabert, C., Katou, Y., Wu, S., Han, J., Wehr, T., Xiao, J., He, X., Zhang, Z., Shi, Y., Shirahige, K., Pasero, P., Shen, X.

Topoisomerase I suppresses genomic instability by preventing interference between replication and transcription.

Tuduri S, Crabbé L, Conti C, Tourrière H, Holtgreve-Grez H, Jauch A, Pantesco V, De Vos J, Thomas A, Theillet C, Pommier Y, Tazi J, Coquelle A, Pasero P.

Single-molecule analysis of DNA replication in yeast and in human cells.

Alabert, C., Poveda, A. and Pasero, P

Specific function of phosphoinositide 3-kinase beta in the control of DNA replication.

Marqués, M., Kumar, A., Poveda, AM., Zuluaga, S., Hernández, C., Jackson, S., Pasero, P., Carrera, AC.

The MRX complex stabilizes the replisome independently of the S phase checkpoint during replication stress.

Tittel-Elmer M, Alabert C, Pasero P, Cobb JA.

Differential regulation of homologous recombination at DNA breaks and replication forks by the Mrc1 branch of the S-phase checkpoint.

Alabert C, Bianco JN, Pasero P

Rtt101 and Mms1 in budding yeast form a CUL4(DDB1)-like ubiquitin ligase that promotes replication through damaged DNA

Zaidi, IW., Rabut, G., Poveda, A., Scheel, H., Malmström, J., Ulrich, H., Hofmann, K., Pasero, P., Peter, M., Luke, B.

A journey into the nucleus. Conference on Nuclear Structure and Dynamics.

Solovei, I., Pasero, P., Visa, N.

Phosphorylation of Slx4 by Mec1 and Tel1 regulates the single-strand annealing mode of DNA repair in budding yeast

Flott, S., Alabert, C., Toh, GW., Toth, R., Sugawara, N., Campbell, DG., Haber, JE., Pasero, P., Rouse, J.

Upregulation of error-prone DNA polymerases beta and kappa slows down fork progression without activating the replication checkpoint

Pillaire MJ, Betous R, Conti C, Czaplicki J, Pasero P, Bensimon A, Cazaux C, Hoffmann JS.

Anaphase onset before complete DNA replication with intact checkpoint responses

Torres-Rosell J, De Piccoli G, Cordon-Preciado V, Farmer S, Jarmuz A, Machin F, Pasero P, Lisby M, Haber JE, Aragon L.

Maintenance of fork integrity at damaged DNA and natural pause sites.

Tourriere, H., Pasero Ph.

An essential role for Orc6 in DNA replication through maintenance of pre-replicative complexes

Semple JW, Da-Silva LF, Jervis EJ, Ah-Kee J, Al-Attar H, Kummer L, Heikkila JJ, Pasero P, Duncker BP.

The cullin Rtt101p promotes replication fork progression through damaged DNA and natural pause sites.

Luke, B, Versini, G, Jaquenoud, M, Zaidi, IW, Kurz, T, Pintard, L, Pasero, P, Peter, M.

Mrc1 and tof1 promote replication fork progression and recovery independently of Rad53

Tourriere, H., Versini, G., Cordon-Preciado, V., Alabert, C., Pasero, P.

Thèses et hdr

Study of the role of RNases H proteins in the cellular response to replication stress 14/04/2022

Defended by Samira Kemiha on 14/04/2022 under the supervision of Armelle Lengronne and Jérôme Poli 

HDR 08/04/2022

How do cells coordinate replication and transcription?
By Jérôme Poli on 08/04/2022

Study of the causes and consequences of endogenous replicative stress in the yeast Saccharomyces cerevisiae 15/11/2019

Defended by Romain Forey on 15/11/2019 under the supervision of Philippe Pasero

Study of the role of SAMHD1 in the replicative stress response and type I interferon production 19/09/2018

Defended by Flavie Coquel on 19/09/2018 under the supervision of Yea-Lih Lin and Philippe Pasero

Genomic study of the interference between replication and transcription as a source of replicative stress 24/11/2017

Defended by Ismaël Padioleau on 24/11/2017 under the supervision of Philippe Pasero

Involvement of replication-transcription interference during cancer development 15/12/2016

Defended by Alexy Promonet on 15/12/2016 under the supervision of Yea-Lih Lin and Philippe Pasero

ALABERT Constance
ALABERT Constance
Chercheur
CHAMI Bayan
CHAMI Bayan
Chercheur
COQUEL Flavie
COQUEL Flavie
Chercheur
CRABBE Laure
CRABBE Laure
Chercheur
GILBERT Nicolas
GILBERT Nicolas
Chercheur
GOPAUL Diyavarshini
GOPAUL Diyavarshini
Chercheur
HASSEN Wafa
HASSEN Wafa
Chercheur
KASSAMBARA Alboukadel
KASSAMBARA Alboukadel
Chercheur
LARCHER Mélanie
LARCHER Mélanie
Chercheur
LE CLECH Mikael
LE CLECH Mikael
Chercheur
MORIEL-CARRETERO Maria
MORIEL-CARRETERO Maria
Chercheur
POVEDA Ana maria
POVEDA Ana maria
Chercheur
SAPEDE Elena
SAPEDE Elena
Chercheur
TECHER Herve
TECHER Herve
Chercheur
TITTEL-ELMER Mireille
TITTEL-ELMER Mireille
Chercheur
TOURRIERE Hélène
TOURRIERE Hélène
Chercheur
TUDURI Sandie
TUDURI Sandie
Chercheur
VERSINI Gwennaelle
VERSINI Gwennaelle
Chercheur
YOSHIDA Kazumasa
YOSHIDA Kazumasa
Chercheur
BIANCO Julien
BIANCO Julien
Doctorant
CHAPTAL Laura
CHAPTAL Laura
Doctorant
DA MOTA Mégane
DA MOTA Mégane
Doctorant
DELAMARRE Axel
DELAMARRE Axel
Doctorant
FOREY Romain
FOREY Romain
Doctorant
HERVIOU Laurie
HERVIOU Laurie
Doctorant
KEMIHA Samira
KEMIHA Samira
Doctorant
PADIOLEAU Ismaël
PADIOLEAU Ismaël
Doctorant
SILVA Maria-joao
SILVA Maria-joao
Doctorant
VIKOVA Veronika
VIKOVA Veronika
Doctorant
VIZITEU Elena
VIZITEU Elena
Doctorant
YANG Chun-Yen
YANG Chun-Yen
Doctorant
YANG Chun-Yen
YANG Chun-Yen
Doctorant
BACAL Julien
BACAL Julien
ITA
BOISSIERE Thierry
BOISSIERE Thierry
ITA
DESMARAIS Damien
DESMARAIS Damien
ITA
DJELEHOUNDE Didier
DJELEHOUNDE Didier
ITA
JOURDAN Michel
JOURDAN Michel
ITA
KONG-HAP Marie
KONG-HAP Marie
ITA
KUMANSKI Sylvain
KUMANSKI Sylvain
ITA
MAURIN Anne-Lyne
MAURIN Anne-Lyne
ITA
MOXLEY Alexandra
MOXLEY Alexandra
ITA
NEGREL Aurélie
NEGREL Aurélie
ITA
THIBAUT Coraline
THIBAUT Coraline
ITA
TORáN Alba
TORáN Alba
ITA
VERNET Audrey
VERNET Audrey
ITA