BENJAMIN PARDO

 

Ph.D. (Biology)

Associate researcher, CNRS. Institute of Human Genetics UMR 9002 CNRS and University of Montpellier, team "Maintenance of genome integrity during replication" directed by Dr. Philippe Pasero (DR1 INSERM).

 

ACADEMIC BACKGROUND

2003 -2006 University of Paris XI. Ph.D. thesis, "Study of the protection of telomere integrity in the yeast Saccharomyces cerevisiae"

1998-2003 University of Paris XI. Bachelor degree. (Magistère de Biotechnologies)

 

RESEARCH EXPERIENCE

2014-present    recruitment CR2 at CNRS in the team "Maintenance of genome integrity during replication" led by Dr. Philippe Pasero (Institute of Human Genetics, Montpellier, France).

                        Project: study of DNA damage repair and signaling mechanisms during S phase in the yeast Saccharomyces cerevisiae.

2012-2014        2nd post-doctoral internship in the team "Maintenance of genome integrity during replication" led by Dr. Philippe Pasero (Institut de Génétique Humaine, Montpellier, France).

                        Project: study of the restart of DNA replication forks by the homologous recombination mechanism in the yeast Saccharomyces cerevisiae.

2007-2012        1st post-doctoral internship in the team "Genome stability & Cancer" directed by Pr. Andrés Aguilera (Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Seville, Spain).

                        Project: Study of the involvement of DNA double-strand break repair mechanisms in the formation of chromosomal translocations in the yeast Saccharomyces cerevisiae.

2002-2007        D.E.A. and Ph.D. thesis in the team "Telomeres and DNA repair" directed by Dr. Stéphane Marcand (Commissariat à l'Energie Atomique, Fontenay aux Roses, France).

                        Project: study of the protection of telomere integrity in the yeast Saccharomyces cerevisiae.

2002                Compulsory Master's internship (6 months) in the team of Pr. Léa Brakier-Gingras (University of Montreal, Canada).

                        Project: study of ribosomal proteins in the bacterium Escherichia coli.

2001                Optional internship (2 months) in the team of Pr. Patrick Forterre (Institut de Génétique et Microbiologie, Université Paris XI, Orsay).

                        Project: study of DNA topoisomerase VI in archaea.

2000                Optional internship (2 months) in the team of Pr. Philippe Silar (Institut de Génétique et Microbiologie, Université Paris XI, Orsay).

                        Project: study of unconventional infectious elements in the fungus Podospora anserina.

 

FUNDINGS

2020                Project leader of an ARC Foundation project (ARC Foundation for Research against Cancer, 2 years). The endowment is 50 k€.

2019                Contributor to the ANR PRC " ReDeFINe " (French Ministry of Research, 4 years). The endowment is 140 k€.

2017                Contributor to the ANR PRC " GeMaPer " (French Ministry of Research, 5 years. The endowment is 174 k€.

2013                Post-doctoral funding contract from the FRM (Foundation for Medical Research, 2 years). The endowment is 110 k€.

2010                Post-doctoral funding contract "Juan de la Cierva" (Spanish Ministry of Research, 2 years). The endowment is 39 k€.

2007                EMBO Long-term Fellowship (European Molecular Biology Organization, 2 years). The endowment is 48 k€.

 

LIST OF PUBLICATIONS

 

Reusswig K-U, Bittmann J, Peritore M, Courtes M, Pardo B, Wierer M, Mann M & Pfander B. (2022) Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions. Nature Communications 13:7014.

Ovejero S, Kumanski S, Soulet C, Azarli J, Pardo B, Santt O, Constantinou A, Pasero P & Moriel Carretero M. (2022) A Sterol-PI(4)P exchanger controls the Tel1/ATM axis of the DNA Damage Response. bioRxiv 2022.07.13.499867

Pourali P, Pasero P & Pardo B $ (2022) Top1p targeting by Fob1p at the ribosomal Replication Fork Barrier does not account for camptothecin sensitivity in Saccharomyces cerevisiae cells. microPublication Biol 2022.

$ Corresponding author

Coiffard J,  Santt O,  Kumanski S,  Pardo B $ &  Moriel-Carretero M $. (2021). A CRISPR-Cas9-based system for the dose-dependent study of DNA double strand breaks sensing and repair. bioRxiv

2021.10.21.465387; doi: https://doi.org/10.1101/2021.10.21.465387

$ Corresponding authors

Pardo B $, Moriel-Carretero M, Vicat T, Aguilera A $ & Pasero P. (2020) A new role of the Mus81 endonuclease for replication completion after fork restart. EMBO Reports 21(7):e49367.

$ Corresponding authors

Forey R, Poveda A, Sharma S, I Padioleau, Renard C, Lambert R, Skrypczak M, Ginalski K, A Lengronne A, Chabes A, Pardo B $ & Pasero P $. (2020) Mec1 is activated at the onset of normal S phase by low dNTP pools impeding DNA replication. Molecular Cell78(3):396-410.e4.

$ Corresponding authors

Voordeckers K, Colding C, Grasso L, Pardo B, Hoes L, Kominek J, Gielens K, Van Loo P, Nuyts S, Pasero P, Lisby M & Verstrepen KJ. (2020) Ethanol exposure increases mutation rate through error-prone polymerases. Nature Communications 11(1):3664.

Zhu Y, Biernacka A, Pardo B, Dojer N, Forey R, Skrzypczak M, Fongang B, Nde J, Yousefi R, Pasero P, Ginalski K & Rowicka M. (2019) qDSB-Seq is a general method for genome-wide quantification of DNA double-strand breaks using sequencing. NatureCommunications 10(1):2313.

Moriel-Carretero M, Pasero P & Pardo B $. (2018) DRC Inc. One business, two associates. Current Genetics, 10.1007/s00294-018-0908-7.

$ Corresponding author

Biernacka #, A., Zhu Y #, Skrypczak M, Forey R, Pardo B, Grzelak M, Nde J, Mitra A, Kudlicki A, Crosetto N, Pasero P, Rowicka M & Ginalski K. (2018) i-BLESS is an ultra-sensitive method for detection of DNA double-strand breaks, Communications Biology 1:181. # equal contribution

Bacal J #, Moriel-Carretero M #, Pardo B, Barthe A, Sharma S, Chabes A, Lengronne A & Pasero P. (2018) Mrc1 and Rad9 cooperate to regulate initiation and elongation of DNA replication in response to DNA damage, The EMBO Journal 37(21). # equal contribution

Muñoz-Galvan S, Garcia-Rubio M, Ortega P, Ruiz JF, Jimeno S, Pardo B, Gomez-Gonzalez B & Aguilera A. (2017) A new role for Rrm3 in repair of replication-born DNA breakage by sister-chromatid recombination, PLoS Genetics 13(5):e1006781. 

Pardo, B., Crabbe, L. & Pasero, P. (2017) Signaling pathways of replication stress in yeast, Fems Yeast Research 1; 17(2).

Ruiz JF #,  Pardo B #, Sastre-Moreno G, Aguilera A & Blanco L. (2013) Yeast Pol4 promotes Tel1-regulated chromosomal translocations, PLoS Genetics 9(7):e1003656. # equal contribution

Pardo B & Aguilera A. (2012) Complex chromosomal rearrangements mediated by break-induced replication and half-crossovers involve structure-selective endonucleases, PLoS Genetics 8(9):e1002979.

Pardo B, Gomez-Gonzalez B & Aguilera A. (2009) DNA repair in mammalian cells: DNA double-strand break repair: how to fix a broken relationship, Cellular & Molecular Life Sciences 66, 1039-56.

Marcand S, Pardo B, Gratias A, Cahun S & Callebaut I (2008) Multiple pathways inhibit NHEJ at telomeres, Genes & Development 22, 1153-8.

Pardo B, Ma E & Marcand S. (2006) Mismatch tolerance by DNA polymerase Pol4 in the course of nonhomologous end joining in Saccharomyces cerevisiae, Genetics 172, 2689-94.

Pardo B & Marcand S. (2005) Rap1 prevents telomere fusions by nonhomologous end joining, The EMBO Journal 24 (17), 3117-27.

 

 

SCIENTIFIC COMMUNICATIONS (2017-2022)

 

2022                EMBO meeting “The DNA damage response in Physiology and Disease”, Sounio, Greece.

Poster: “Coordination between the S-phase checkpoint regulation and replication fork repair by Fun30 and Slx4”.

                        Meeting “3R : Réplication, Recombinaison & Réparation”, Giens, France.

                        Poster: “DNA damage-induced Transcription/Replication conflicts are at the basis of the cytotoxicity of the antitumoral drug camptothecin”.

 

2020                Meeting “5^th French-German DNA Repair Meeting”, Montpellier, France.

                        Talk : “Homologous recombination and Mus81 promote replication completion in response to replication blockage”

                        Meeting “Trends in Genome Integrity and Genome Dynamics”, Seville, Spain.

                        Poster: “Mec1^ATR is activated at the onset of S phase by low dNTP pools impeding DNA replication”

 

 

2019                Meeting “EMBO YIP Sectoral meeting on Genome Integrity”, Zurich, Switzerland.

                        Talk : “Kinetics of the checkpoint response to replication stress”

                        Meeting “3R : Réplication, Recombinaison & Réparation”, Giens, France.

                        Poster: “Mec1 is activated at the onset of normal S phase by low dNTP pools impeding DNA replication”

 

2018                Meeting “Club Noyau”, Paris, France.

                        Talk : “Yeast cells require endogenous stress to carry out DNA replication”

                        Congrès “FASEB Dynamic DNA Structures in Biology”, Olean, U.S.A.

                        Présentation orale: “Checkpoint control of endogenous replication stress”

 

2017                Meeting “EMBO YIP Sectoral meeting on Genome Integrity”, Heraklion, Greece.

                        Talk : “The replication checkpoint is activated during an unchallenged S phase: why and what for?”

                        Meeting “3R : Réplication, Recombinaison & Réparation”, Giens, France.

                        Poster: “Repair and Signaling of Camptothecin-induced DNA damage”