Genome Surveillance and Stability

Molecular bases of human diseases

• Team
• Projects
• Members
• Publications
• Theses and hdr

https://www.igh.cnrs.fr/uploads/media/66707c191457e/2024-dm-lab-photo-cropped.jpeg

Our group is generally interested in the regulation of the DNA Damage Response (DDR), and in particular in its regulation during the early steps of embryonic development. The main function of the DDR is to slow down, or arrest cell prolferation in the presence of DNA lesions (DNA breaks, telomer integrity, replication forks stall) so to avoid cell division in the presence of DNA damage and therefore avoid the propagation of mutations that are drivers of genomic instability. A strong genomic instability is a feature of cancer cells (telomeric fusions, trabnsocations, duplications, deletions). To date it is admitted that the majority of sporadic tumours are the consequence of mutations in DDR genes, which can also lead to the development of genetic diseases. Hence the DDR is currently considered as a major barrier to malignat transformation playing a key role in maintenace of genomic stability.

 

Waqar ALI
Researcher
0434359973

Antoine AZE
Researcher
0434359973

James HUTCHINS
Researcher
0434359973

Mouhamed IDRISSOU
Researcher
0411759728

Domenico MAIORANO
Researcher
0434359946

Nathalie MALIRAT
ITA
0434359973

Publications of the team

A non-catalytic function for Rad18 in sustaining glioblastoma proliferation

Published the 10/09/2024 - BioRxiv

Chames Kermi, Nour Benbahouche, Lenka Stefancikova, Aurore Siegfried, Jean-Marc Pascussi, Julie Pannequin, Jérôme Moreaux, Tom Egger, Jean-Philippe Hugnot, Marie-Bernadette Delisle, Elizabeth Moyal, Emmanuelle Uro-Coste, Domenico Maiorano

more

Detection of endogenous translesion DNA synthesis in single mammalian cells

Published the 14/06/2023 - Cell Reports Methods 37426760

Egger T, Aze A, Maiorano D

Identification and Characterisation of Proteins Binding to a G-Quadruplex Origin G-rich Repeated Element in Mammalian Cells

Published the 31/03/2023 - BioRxiv

James R. A. Hutchins, Isabelle Peiffer, Serge Urbach, Jean-Louis Mergny, View ORCID ProfilePhilippe Marin, Domenico Maiorano, Marcel Méchali

Translesion DNA synthesis-driven mutagenesis in very early embryogenesis of fast cleaving embryos

Published the 25/01/2022 - Nucleic Acids Research

Lo Furno E., Busseau I., Aze A., Lorenzi C., Saghira C., Danzi M., Zuchner S., Maiorano D.

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Studying the DNA damage response in embryonic systems.

Published the 16/11/2021 - Methods Enzymol , 661:95-120 34776225

Lo Furno E, Recolin B, van der Laan S, Aze A, Maiorano D

Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress.

Published the 10/04/2021 - Int J Mol Sci , 22(8) 33920223

Maiorano D, El Etri J, Franchet C, Hoffmann JS

Dihydropyrimidinase protects from DNA replication stress caused by cytotoxic metabolites

Published the 28/02/2020 - Nucleic Acids Research 31853544

Jihane Basbous, Antoine Aze, Laurent Chaloin, Rana Lebdy, Dana Hodroj, Cyril Ribeyre, Marion Larroque, Caitlin Shepard, Baek Kim, Alain Pruvost, Jérôme Moreaux, Domenico Maiorano, Marcel Mechali, Angelos Constantinou

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Preserving Genome Integrity During the Early Embryonic DNA Replication Cycles.

Published the 24/05/2019 - Genes (Basel) , 10(5) 31137726

Kermi C, Aze A, Maiorano D

more

Recent advances in understanding DNA replication: cell type-specific adaptation of the DNA replication program.

Published the 20/09/2018 - F1000Res , 7 30228862

Aze A, Maiorano D

+ Replication and Genome Dynamics

Ddx19 links mRNA nuclear export with progression of transcription and replication and suppresses genomic instability upon DNA damage in proliferating cells

Published the 01/01/2017 - Nucleus , 8, 5, 1-7 28696814

Hodroj, D., Serhal, K., Maiorano, D.

An ATR-dependent function for the Ddx19 RNA helicase in nuclear R-loop metabolism

Published the 01/01/2017 - EMBO J. , 36(9):1182-1198 28314779

Hodroj D, Recolin B, Serhal K, Martinez S, Tsanov N, Abou Merhi R, Maiorano D

Regulation of DNA Replication in Early Embryonic Cleavages

Published the 01/01/2017 - Genes , 8,1, 42 28106858

Kermi, C., Lo Furno, E., Maiorano, D

Reversible p53 inhibition prevents cisplatin ototoxicity without blocking chemotherapeutic efficacy

Published the 01/01/2017 - EMBO Mol Med. , 9, 1, 7-26 27794029

Benkafadar N, Menardo J, Bourien J, Nouvian R, François F, Decaudin D, Maiorano D, Puel JL, Wang J.

Chapter 6 -Open Access - Genomic instability of pluripotent stem cells: origins and consequences

Published the 01/01/2016 - , 119-137 - edited by Minoru Tomizawa, ISBN 978-953-51-2472-6, Print ISBN 978-953-51-2471-9, Published

Lo Furno, E., van der Laan, S., and Maiorano, D.

Rad18 is a maternal limiting factor that suppresses the UV-dependent DNA damge checkpoint in Xenopus embryos

Published the 01/01/2015 - Developmental Cell , 34(3):364-372 26212134

Kermi, C., Prieto, S., van der Laan, S., Tsanov, N., Recolin, B., Uro-Coste, E., Delisle, M-B., and Maiorano, D.

Regulation of translesion DNA synthesis by PCNA monoubiquitylation and beyond

Published the 01/01/2015 - Function of Translesion DNA polymerases, ISBN: 978-81-308-0538-2. Editors: Domenico Maiorano & Jean-Sébastien Hoffmann. , 127-152

Tsanov, N., Kermi, C., Delgado, J., Serrano, L., Maiorano D.

Checking the cycle by ERRβ splice variants

Published the 01/01/2015 - Cell Cycle , 14(10):1492-3 25789879

Vanacker JM, Maiorano D.

Molecular mechanisms of DNA replication checkpoint activation

Published the 01/01/2014 - Genes (Basel) , 5,1, 147-175 24705291

Recolin B, van der Laan S, Tsanov N, Maiorano D.

Cell Cycle-Dependent Expression of Dub3, Nanog and the p160 Family of Nuclear Receptor Coactivators (NCoAs) in Mouse Embryonic Stem Cells

Published the 01/01/2014 - PLoS One , 9(4):e93663 24695638

van der Laan S, Golfetto E, Vanacker JM, Maiorano D.

Post-translational modifications in embryonic cell cycle

Published the 01/01/2014 - Cell Cycle , 13(9) 24698793

Van der Laan, S., Maiorano, D.

PIP degron proteins, substrates of CRL4Cdt2, and not PIP boxes, interfere with DNA polymerase h and k focus formation upon UV damage

Published the 01/01/2014 - Nucleic Acids Research , 42, 6, 3692-3706

Tsanov, N., Kermi, C., Coulombe, P., Van der Laan, S., Hodroj, D., Maiorano, D.

High Dub3 expression in mouse ESC couples the G1/S checkpoint to pluripotency

Published the 01/01/2013 - Molecular Cell , 52, 3, 366-379 24207026

Van der Laan, S., Crozet, C., Tsanov, N., and Maiorano, D

Polk in replication checkpoint

Published the 01/01/2013 - Cell Cycle , 12, 24, 3713-3714 24189533

Maiorano, D., Hoffmann, JS.

DNA polymerase k-dependent DNA synthesis at stalled replication forks is important for Chk1 activation

Published the 01/01/2013 - EMBO Journal , 32(15):2172-85 23799366

Bétous R., Pillaire, M-J, Pierini, L., Van der Laan, S., Recolin B., Ohl-Séguy, E., Guo, C., Niimi, N., Gruz, P., Nohmi, T. Friedberg, E., Cazaux, C., Maiorano, D* and Hoffmann J-S*. * corresponding authors

Role of RPA as sensor in activation of the S-phase checkpoint in Xenopus egg extracts

Published the 01/01/2012 - Nucleic Acids Research , 40(8):3431-42, doi: 10.1093/nar/gkr1241 22187152

Recolin, B., Van der Laan, S., and Maiorano, D.

Implication of RPA32 phosphorylation in S-phase checkpoint signalling at replication forks stalled with aphidicolin in Xenopus egg extracts

Published the 01/01/2012 - Biochemical and Biophysical Research Communications , 427, 785-789, pii: S0006-291X(12)01917-1. doi: 10.1016/j.bbrc.2012.09.139

Recolin, B., Maiorano, D

Regulation of S phase during early embryonic Xenopus development

Published the 01/01/2009 - In « Cell Cycle and Development in vertebrates ». Ed. Research Signpost

Recolin, B., Maiorano, D.

XRCC1 interacts with the p58 subunit of DNA Pola-primase and may coordinate DNA repair and replication during S phase.

Published the 01/01/2009 - Nuclei Acids Research , 37, 10, 3177-3188 19305001

Levy N, Oehlmann M, Delalande F, Nasheuer HP, Van Dorsselaer A, Schreiber V, De Murcia G, Ménissier-de Murcia J, Maiorano D, Bresson Anne.

Geminin is cleaved by caspase-3 during apoptosis in Xenopus egg extracts

Published the 01/01/2007 - Biochem Biophys Res Commun. , 361, 2, 276-280 17651691

Auziol C, Mechali M, Maiorano D.

+ Replication and Genome Dynamics

A Cdt1-geminin complex licenses chromatin for DNA replication and prevents rereplication during S phase in Xenopus.

Published the 01/01/2006 - EMBO J. , 25(24):5764-74 17124498

Lutzmann M, Maiorano D, Mechali M.

+ Replication and Genome Dynamics

MCM proteins and DNA replication.

Published the 01/01/2006 - Curr Opin Cell Biol. , 18, 130-136 16495042

Maiorano D, Lutzmann M, Mechali M.

+ Replication and Genome Dynamics

Identification of full genes and proteins of MCM9, a novel, vertebrate-specific member of the MCM2-8 protein family

Published the 01/01/2005 - Gene , 362:51-6 16226853

Lutzmann, M., Maiorano, D., and Méchali, M.

+ Replication and Genome Dynamics

MCM8, a novel DNA helicase which is not required for licensing but functions during processive chromosomal replication in vertebratres.

Published the 01/01/2005 - Cell , 120, 315-3128

Maiorano, D., Cuvier, O., Danis, E., and Mechali, M.

+ Replication and Genome Dynamics

Recombinant Cdt1 induces rereplication of G2 nuclei in Xenopus egg extracts

Published the 01/01/2005 - Current Biology , 15, p 146-153

Maiorano, D., Krasinska, L., Lutzmann, M. and Mechali M.

+ Replication and Genome Dynamics

A hypophosphorylated form of RPA34 is a specific component of pre-replication centers.

Published the 01/01/2004 - J Cell Science , 117, p 4909-4920

Françon, P. ; Lemaitre, JM., Dreyer, C. ; Maiorano, D. ; Cuvier, O. and Marcel Méchali.

+ Replication and Genome Dynamics

Crystal Structure of the Coiled-coil Dimerization Motif of Geminin : Structural and Functional Insights on DNA Replication Regulation.

Published the 01/01/2004 - J Mol Biol. , 342, 275-287

Thepaut, M., Maiorano, D., Guichou, JF., Auge, MT., Dumas, C., Méchali, M., and Padilla, A.

+ Replication and Genome Dynamics

Specification of a DNA replication origin by a transcription complex.

Published the 01/01/2004 - Nature Cell Biology , 6, 721-730. This article has been the subject of an Editors

Danis, E., Brodolin, K., Menut, S., Maiorano, D., Girard-Reydet, C. and Marcel Méchali.

+ Replication and Genome Dynamics

Cell cycle regulation of the licensing activity of Cdt1 in Xenopus laevis.

Published the 01/01/2004 - Experimental Cell research , 295, 138-149

Maiorano, D., Rul, W., and Marcel Mechali

+ Replication and Genome Dynamics

Many roads lead to the origin

Published the 01/01/2002 - Nature Cell Biology , 4, E58-E59

Maiorano, D., and Méchali, M.

+ Replication and Genome Dynamics

Repression of origin assembly in metaphase depends on inhibition of RLF-B/cdt1 by geminin.

Published the 01/01/2001 - Nature Cell Biology , 3, 107-113

Tada, S., Li, A., Maiorano, D., Méchali, M., and Blow, J.

+ Replication and Genome Dynamics

XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis

Published the 01/01/2000 - Nature , 404, 622-625 ( cf also News and Views 404, 560-561)

Maiorano, D., Moreau, J., and Méchali, M.

+ Replication and Genome Dynamics

Stepwise Regulated Chromatin Assembly of MCM2-7 Proteins.

Published the 01/01/2000 - J. Biol. Chem. , 275, 8426-8431

Maiorano, D., Lemaître, J.M. and Méchali, M.

+ Replication and Genome Dynamics

Initiation of DNA replication in eukaryotes : questioning the origin.

Published the 01/01/1999 - Minireview FEBS Letters , 452, 87-91.

Françon, P., Maiorano, D. and Méchali, M.

+ Replication and Genome Dynamics

Evidence for different MCM subcomplexes with differential binding to chromatin in Xenopus.

Published the 01/01/1998 - Experimental Cell Research , 245, 282-289.

Coué, M., Amariglio, F., Maiorano, D., Bocquet, S. and Méchali, M.

+ Replication and Genome Dynamics

Thèses et hdr

Functional characterization of the tolerance to DNA damage of early embryosSubject: 25/11/2019

Defended by Elena Lo Furno on 25/11/2019 under the supervision of Domenico Maiorano

 

Functional interaction between the lesion tolerance system and the DNA damage checkpoint: implications for genome stability and oncogenesis 14/12/2016

Defended by Chames Kermi on 14/12/2016 under the supervision of Domenico Maiorano