Translesion DNA synthesis-driven mutagenesis in very early embryogenesis of fast cleaving embryos
Lo Furno E., Busseau, I., Aze, A., Lorenzi, C., Saghira, C., Danzi, M., Zuchner, S., Maiorano, D.
Genome Surveillance and Stability
Molecular bases of human diseases
Our group is generally interested in the regulation of the DNA Damage Response (DDR), and in particular in its regulation during the early steps of embryonic development. The main function of the DDR is to slow down, or arrest cell prolferation in the presence of DNA lesions (DNA breaks, telomer integrity, replication forks stall) so to avoid cell division in the presence of DNA damage and therefore avoid the propagation of mutations that are drivers of genomic instability. A strong genomic instability is a feature of cancer cells (telomeric fusions, trabnsocations, duplications, deletions). To date it is admitted that the majority of sporadic tumours are the consequence of mutations in DDR genes, which can also lead to the development of genetic diseases. Hence the DDR is currently considered as a major barrier to malignat transformation playing a key role in maintenace of genomic stability.
Publications of the team
Studying the DNA damage response in embryonic systems.
Lo Furno E, Recolin B, van der Laan S, Aze A, Maiorano D
Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress.
Maiorano D, El Etri J, Franchet C, Hoffmann JS
Dihydropyrimidinase protects from DNA replication stress caused by cytotoxic metabolites
Jihane Basbous, Antoine Aze, Laurent Chaloin, Rana Lebdy, Dana Hodroj, Cyril Ribeyre, Marion Larroque, Caitlin Shepard, Baek Kim, Alain Pruvost, Jérôme Moreaux, Domenico Maiorano, Marcel Mechali, Angelos Constantinou
Recent advances in understanding DNA replication: cell type-specific adaptation of the DNA replication program.
Aze A, Maiorano D
Reversible p53 inhibition prevents cisplatin ototoxicity without blocking chemotherapeutic efficacy
Benkafadar N, Menardo J, Bourien J, Nouvian R, François F, Decaudin D, Maiorano D, Puel JL, Wang J.
Regulation of DNA Replication in Early Embryonic Cleavages
Kermi, C., Lo Furno, E., Maiorano, D
An ATR-dependent function for the Ddx19 RNA helicase in nuclear R-loop metabolism
Hodroj D, Recolin B, Serhal K, Martinez S, Tsanov N, Abou Merhi R, Maiorano D
Ddx19 links mRNA nuclear export with progression of transcription and replication and suppresses genomic instability upon DNA damage in proliferating cells
Hodroj, D., Serhal, K., Maiorano, D.
Chapter 6 -Open Access - Genomic instability of pluripotent stem cells: origins and consequences
Lo Furno, E., van der Laan, S., and Maiorano, D.
Regulation of translesion DNA synthesis by PCNA monoubiquitylation and beyond
Tsanov, N., Kermi, C., Delgado, J., Serrano, L., Maiorano D.
Rad18 is a maternal limiting factor that suppresses the UV-dependent DNA damge checkpoint in Xenopus embryos
Kermi, C., Prieto, S., van der Laan, S., Tsanov, N., Recolin, B., Uro-Coste, E., Delisle, M-B., and Maiorano, D.
Checking the cycle by ERRβ splice variants
Vanacker JM, Maiorano D.
PIP degron proteins, substrates of CRL4Cdt2, and not PIP boxes, interfere with DNA polymerase h and k focus formation upon UV damage
Tsanov, N., Kermi, C., Coulombe, P., Van der Laan, S., Hodroj, D., Maiorano, D.
Post-translational modifications in embryonic cell cycle
Van der Laan, S., Maiorano, D.
Cell Cycle-Dependent Expression of Dub3, Nanog and the p160 Family of Nuclear Receptor Coactivators (NCoAs) in Mouse Embryonic Stem Cells
van der Laan S, Golfetto E, Vanacker JM, Maiorano D.
Molecular mechanisms of DNA replication checkpoint activation
Recolin B, van der Laan S, Tsanov N, Maiorano D.
DNA polymerase k-dependent DNA synthesis at stalled replication forks is important for Chk1 activation
Bétous R., Pillaire, M-J, Pierini, L., Van der Laan, S., Recolin B., Ohl-Séguy, E., Guo, C., Niimi, N., Gruz, P., Nohmi, T. Friedberg, E., Cazaux, C., Maiorano, D* and Hoffmann J-S*. * corresponding authors
Polk in replication checkpoint
Maiorano, D., Hoffmann, JS.
High Dub3 expression in mouse ESC couples the G1/S checkpoint to pluripotency
Van der Laan, S., Crozet, C., Tsanov, N., and Maiorano, D
Role of RPA as sensor in activation of the S-phase checkpoint in Xenopus egg extracts
Recolin, B., Van der Laan, S., and Maiorano, D.
Implication of RPA32 phosphorylation in S-phase checkpoint signalling at replication forks stalled with aphidicolin in Xenopus egg extracts
Recolin, B., Maiorano, D
XRCC1 interacts with the p58 subunit of DNA Pola-primase and may coordinate DNA repair and replication during S phase.
Levy N, Oehlmann M, Delalande F, Nasheuer HP, Van Dorsselaer A, Schreiber V, De Murcia G, Ménissier-de Murcia J, Maiorano D, Bresson Anne.
Regulation of S phase during early embryonic Xenopus development
Recolin, B., Maiorano, D.
Geminin is cleaved by caspase-3 during apoptosis in Xenopus egg extracts
Auziol C, Mechali M, Maiorano D.
MCM proteins and DNA replication.
Maiorano D, Lutzmann M, Mechali M.
+
Replication and Genome Dynamics
A Cdt1-geminin complex licenses chromatin for DNA replication and prevents rereplication during S phase in Xenopus.
Lutzmann M, Maiorano D, Mechali M.
+
Replication and Genome Dynamics
Recombinant Cdt1 induces rereplication of G2 nuclei in Xenopus egg extracts
Maiorano, D., Krasinska, L., Lutzmann, M. and Mechali M.
+
Replication and Genome Dynamics
MCM8, a novel DNA helicase which is not required for licensing but functions during processive chromosomal replication in vertebratres.
Maiorano, D., Cuvier, O., Danis, E., and Mechali, M.
+
Replication and Genome Dynamics
Identification of full genes and proteins of MCM9, a novel, vertebrate-specific member of the MCM2-8 protein family
Lutzmann, M., Maiorano, D., and Méchali, M.
+
Replication and Genome Dynamics
A hypophosphorylated form of RPA34 is a specific component of pre-replication centers.
Françon, P. ; Lemaitre, JM., Dreyer, C. ; Maiorano, D. ; Cuvier, O. and Marcel Méchali.
+
Replication and Genome Dynamics
Crystal Structure of the Coiled-coil Dimerization Motif of Geminin : Structural and Functional Insights on DNA Replication Regulation.
Thepaut, M., Maiorano, D., Guichou, JF., Auge, MT., Dumas, C., Méchali, M., and Padilla, A.
+
Replication and Genome Dynamics
Cell cycle regulation of the licensing activity of Cdt1 in Xenopus laevis.
Maiorano, D., Rul, W., and Marcel Mechali
+
Replication and Genome Dynamics
Specification of a DNA replication origin by a transcription complex.
Danis, E., Brodolin, K., Menut, S., Maiorano, D., Girard-Reydet, C. and Marcel Méchali.
+
Replication and Genome Dynamics
Repression of origin assembly in metaphase depends on inhibition of RLF-B/cdt1 by geminin.
Tada, S., Li, A., Maiorano, D., Méchali, M., and Blow, J.
+
Replication and Genome Dynamics
Stepwise Regulated Chromatin Assembly of MCM2-7 Proteins.
Maiorano, D., Lemaître, J.M. and Méchali, M.
+
Replication and Genome Dynamics
XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis
Maiorano, D., Moreau, J., and Méchali, M.
+
Replication and Genome Dynamics
Initiation of DNA replication in eukaryotes : questioning the origin.
Françon, P., Maiorano, D. and Méchali, M.
+
Replication and Genome Dynamics
Evidence for different MCM subcomplexes with differential binding to chromatin in Xenopus.
Coué, M., Amariglio, F., Maiorano, D., Bocquet, S. and Méchali, M.
+
Replication and Genome Dynamics
#}
Thèses
Subject: Molecular characterization of new factors involved in DNA damage tolerance
Hanane Mechri - Start October 2021 Supervisor: Domenico Maiorano Co-supervisor: Antoine Aze
Thesis proposal: Subject: Molecular characterization of new factors involved in DNA damage tolerance
