Gene regulation

Genome dynamics

All organisms must regulate the expression of their genes to achieve the silencing of certain genes, and the activation of others during development and homeostasis. Disregulation of gene expression frequently has dire consequences, and can lead to pathologies such as cancer. The regulation of gene expression occurs at different levels, all of which depend on a multitude of factors. Chromatin is a primary regulator of gene expression. Physical compaction of the genome into chromatin controls accessability to the transcription machinery. Studies performed over recent years have revealed the enormous complexity involved in modifying chromatin to regulate gene expression.

PUBLICATIONS OF THE TEAM

Chromatin-associated MRN complex protects highly transcribing genes from genomic instability

Kader Salifou, Callum Burnard, Poornima Basavarajaiah, Marion Helsmoortel, Victor Mac, David Depierre, Giuseppa Grasso, Céline Franckhauser, Emmanuelle Beyne, Xavier Contreras, Jérôme Dejardin, Sylvie Rouquier, Olivier Cuvier, Rosemary Kiernan

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PAF1 facilitates RNA polymerase II ubiquitination by the Elongin A complex through phosphorylation by CDK12

Gabriel Sanchez, Jérôme Barbier, Céline Elie, Rosemary Kiernan, Sylvie Rouquier

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NF90 modulates processing of a subset of human pri-miRNAs

Grasso G, Higuchi T, Mac V, Barbier J, Helsmoortel M, Lorenzo C, Sanchez G, Bello M, Ritchie W, Sakamoto S, and Kiernan R.

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[Nuclear RNA surveillance complexes control HIV-1 transcription].

Salifou K, Kiernan R, Contreras X

An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression.

Barbier J, Chen X, Sanchez G, Cai M, Helsmoortel M, Higuchi T, Giraud P, Contreras X, Yuan G, Feng Z, Nait-Saidi R, Deas O, Bluy L, Judde JG, Rouquier S, Ritchie W, Sakamoto S, Xie D, Kiernan R

Nuclear RNA surveillance complexes silence HIV-1 transcription.

Contreras X, Salifou K, Sanchez G, Helsmoortel M, Beyne E, Bluy L, Pelletier S, Rousset E, Rouquier S, Kiernan R

Identification of histone 3 variant 2 interacting factors

Latreille D, Bluy L, Benkirane M, Kiernan RE.

Premature termination of transcription by RNAP II: The beginning of the end.

Contreras X, Benkirane M, Kiernan R.

Microprocessor, Setx, Xrn2 and Rrp6 Co-Operate to Induce Premature Termination of Transcription by RNAPII

Wagschal, A., Rousset, E., Basavarajaiah, P., Contreras, X. , Harwig, A., Laurent-Chabalier, S. , Nakamura, M., Chen, X., Zhang, K., Meziane, O., Boyer, F., Parrinello, H., Berkhout, B., Terzian, C., Benkirane, M., Kiernan, R.

Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR

Nakamura M, Basavarajaiah P, Rousset E, Beraud C, Latreille D, Henaoui IS, Lassot I, Mari B, Kiernan R.

PUBLICATIONS COMMUNES

HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP

Sobhian, B., Laguette, N., Yatim, A., Nakamura, M., Levy, Y., Kiernan, R., Benkirane, M.
2010 - Molecular Cell , 38, 3, 439-451 20471949
Service porteur : Laboratory of Molecular Virology

Suv39H1 and HP1gamma are responsible for chromatin-mediated HIV-1 transcriptional silencing and post integration latency.

du Chéné, I., Basyuk, E., Lin,YL., Triboulet, R., Knezevich, A., Chable-Bessia, C., Mettling, C., Baillat, V., Reynes, J., Corbeau, P., Bertrand, E., Marcello, A., Emiliani, S., Kiernan, R., Benkirane, M.
2007 - EMBO.J. , 26(2):424-35. 17245432
Service porteur : Laboratory of Molecular Virology

Intrinsic ubiquitylation activity of PCAF controls the stability of the oncoprotein Hdm2

Linares, Laetitia, Rosemary Kiernan, Robinson Triboulet, Christine Chable-Bessia, Daniel Latreille, Olivier Cuvier, Matthieu Lacroix, Laurent Le Cam, Olivier Coux, and Monsef Benkirane.
2007 - Nature Cell Biology , 9(3):331-338 17293853
Service porteur : Laboratory of Molecular Virology

The Proteasome Regulates HIV-1 Transcription by Both Proteolytic and Non-Proteolytic Mechanisms

Lassot, L., Latreille, D., Rousset, E., Sourisseau, M., Linares, L.K., Chable-Bessia, C., Coux, O., Benkirane, M., Kiernan, R.
2007 - Molecular Cell , 25, 369-383 17289585
Service porteur : Laboratory of Molecular Virology

The HIV-1 Tat protein stimulates reverse transcription in vitro.

Apolloni, A., Meredith, LW., Suhrbier, A., Kiernan, R., Harrich, D
2007 - Curr. HIV Res. , 5 ,5, 473-483 17896967
Service porteur : Laboratory of Molecular Virology

Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter.

Treand C, du Chene I, Bres V, Kiernan R, Benarous R, Benkirane M, Emiliani S.
2006 - EMBO J. , 25, 1690-1699 16601680
Service porteur : Laboratory of Molecular Virology

A non proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter

Vanessa Brès, Rosemary E. Kiernan, Laetitia K. Linares, Christine Chable-Bessia, Olga Plechakova, Céline Tréand, Stephane Emiliani, Jean-Marie Peloponese, Kuan-Teh Jeang, Olivier Coux, Martin Scheffner, and Monsef Benkirane
2003 - Nature Cell Biology , 5, 754-761
Service porteur : Laboratory of Molecular Virology

Tat acetyl-acceptor lysines are important for Human Immunodeficiency Virus type-1 replication

Brès, V., Rosemary Kiernan, Stéphane Emiliani and Monsef Benkirane
2002 - J. Biol. Chem. 277 , 22215-22221
Service porteur : Laboratory of Molecular Virology

Differential acetylation of Tat coordinates its interaction with the co-activators cyclin T1 and PCAF.

Brès V. Tagami H. Péloponèse J.M. Loret E. Jeang K-T. Nakatani Y. Emiliani S. Benkirane M. and Kiernan ER.
2002 - EMBO.J. , 21:6811-6819
Service porteur : Laboratory of Molecular Virology

Interaction between cyclin T1 and SCFSKP2 targets CDK9 for ubiquitination and degradation by the proteasome.

Kiernan, R., Emiliani, S., Nakayama, K., Castro, A., Labbé, J.C., Lorca, T., Nakayama, K.-I., and Benkirane, M.
2001 - Mol. Cell. Biol. , 21 (23), 7956-7970
Service porteur : Laboratory of Molecular Virology

HIV-1 Tat transcriptional activity is regulated by acetylation.

Kiernan, R., Vanhulle, C., Schiltz, L., Adam, E., Xiao, H., Maudoux, F., Calomme, C., Burny, A., Nakatani, Y., Jeang, K.-T., Benkirane, M. and Van Lint, C.
1999 - EMBO J. , 18, 6106-6118.
Service porteur : Laboratory of Molecular Virology

HELSMOORTEL Marion
HELSMOORTEL Marion
BioAster

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SALIFOU Kader
SALIFOU Kader

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GIRAUD Pierre
GIRAUD Pierre

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BEYNE Emmanuelle
BEYNE Emmanuelle

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NOGARET Maguelone
NOGARET Maguelone

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GRASSO Giuseppa
GRASSO Giuseppa

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MAC Victor
MAC Victor

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TANCELIN Mathilde
TANCELIN Mathilde

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BERAUD Cyprien
BERAUD Cyprien

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ASTIER William
ASTIER William
Ecole Polytechnique Fédérale de Lausanne (EPFL), Suisse

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NAKAMURA Mirai
NAKAMURA Mirai

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CHEN Xin
CHEN Xin

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BASAVARAJAIAH Poornima
BASAVARAJAIAH Poornima

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COLENO-COSTES Anne
COLENO-COSTES Anne
IGMM CNRS

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CRUNCHANT Eleonore
CRUNCHANT Eleonore

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PELLETIER Stéphane
PELLETIER Stéphane

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VERLEENE Fantine
VERLEENE Fantine

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Topic: Role of NF90 in the miRNA pathway
Giuseppa Grasso
from October 2017 to October 2021
Supervisor: Rosemary Kiernan