Gene regulation
All organisms must regulate the expression of their genes to achieve the silencing of certain genes, and the activation of others during development and homeostasis. Disregulation of gene expression frequently has dire consequences, and can lead to pathologies such as cancer. The regulation of gene expression occurs at different levels, all of which depend on a multitude of factors. Chromatin is a primary regulator of gene expression. Physical compaction of the genome into chromatin controls accessability to the transcription machinery. Studies performed over recent years have revealed the enormous complexity involved in modifying chromatin to regulate gene expression.
PUBLICATIONS OF THE TEAM
Chromatin-associated MRN complex protects highly transcribing genes from genomic instability
Kader Salifou, Callum Burnard, Poornima Basavarajaiah, Marion Helsmoortel, Victor Mac, David Depierre, Giuseppa Grasso, Céline Franckhauser, Emmanuelle Beyne, Xavier Contreras, Jérôme Dejardin, Sylvie Rouquier, Olivier Cuvier, Rosemary Kiernan
NF90 modulates processing of a subset of human pri-miRNAs
Grasso G, Higuchi T, Mac V, Barbier J, Helsmoortel M, Lorenzo C, Sanchez G, Bello M, Ritchie W, Sakamoto S, and Kiernan R.
[Nuclear RNA surveillance complexes control HIV-1 transcription].
Salifou K, Kiernan R, Contreras X
An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression.
Barbier J, Chen X, Sanchez G, Cai M, Helsmoortel M, Higuchi T, Giraud P, Contreras X, Yuan G, Feng Z, Nait-Saidi R, Deas O, Bluy L, Judde JG, Rouquier S, Ritchie W, Sakamoto S, Xie D, Kiernan R
Nuclear RNA surveillance complexes silence HIV-1 transcription.
Contreras X, Salifou K, Sanchez G, Helsmoortel M, Beyne E, Bluy L, Pelletier S, Rousset E, Rouquier S, Kiernan R
Identification of histone 3 variant 2 interacting factors
Latreille D, Bluy L, Benkirane M, Kiernan RE.
Premature termination of transcription by RNAP II: The beginning of the end.
Contreras X, Benkirane M, Kiernan R.
Microprocessor, Setx, Xrn2 and Rrp6 Co-Operate to Induce Premature Termination of Transcription by RNAPII
Wagschal, A., Rousset, E., Basavarajaiah, P., Contreras, X. , Harwig, A., Laurent-Chabalier, S. , Nakamura, M., Chen, X., Zhang, K., Meziane, O., Boyer, F., Parrinello, H., Berkhout, B., Terzian, C., Benkirane, M., Kiernan, R.
Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR
Nakamura M, Basavarajaiah P, Rousset E, Beraud C, Latreille D, Henaoui IS, Lassot I, Mari B, Kiernan R.
PUBLICATIONS COMMUNES
HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP
Sobhian, B., Laguette, N., Yatim, A., Nakamura, M., Levy, Y., Kiernan, R., Benkirane, M.
2010 - Molecular Cell
, 38, 3, 439-451 20471949 
Service porteur :
Laboratory of Molecular Virology
Suv39H1 and HP1gamma are responsible for chromatin-mediated HIV-1 transcriptional silencing and post integration latency.
du Chéné, I., Basyuk, E., Lin,YL., Triboulet, R., Knezevich, A., Chable-Bessia, C., Mettling, C., Baillat, V., Reynes, J., Corbeau, P., Bertrand, E., Marcello, A., Emiliani, S., Kiernan, R., Benkirane, M.
2007 - EMBO.J.
, 26(2):424-35. 17245432
Service porteur :
Laboratory of Molecular Virology
Intrinsic ubiquitylation activity of PCAF controls the stability of the oncoprotein Hdm2
Linares, Laetitia, Rosemary Kiernan, Robinson Triboulet, Christine Chable-Bessia, Daniel Latreille, Olivier Cuvier, Matthieu Lacroix, Laurent Le Cam, Olivier Coux, and Monsef Benkirane.
2007 - Nature Cell Biology
, 9(3):331-338 17293853
Service porteur :
Laboratory of Molecular Virology
The Proteasome Regulates HIV-1 Transcription by Both Proteolytic and Non-Proteolytic Mechanisms
Lassot, L., Latreille, D., Rousset, E., Sourisseau, M., Linares, L.K., Chable-Bessia, C., Coux, O., Benkirane, M., Kiernan, R.
2007 - Molecular Cell
, 25, 369-383 17289585
Service porteur :
Laboratory of Molecular Virology
The HIV-1 Tat protein stimulates reverse transcription in vitro.
Apolloni, A., Meredith, LW., Suhrbier, A., Kiernan, R., Harrich, D
2007 - Curr. HIV Res.
, 5 ,5, 473-483 17896967
Service porteur :
Laboratory of Molecular Virology
Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter.
Treand C, du Chene I, Bres V, Kiernan R, Benarous R, Benkirane M, Emiliani S.
2006 - EMBO J.
, 25, 1690-1699 16601680
Service porteur :
Laboratory of Molecular Virology
A non proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter
Vanessa Brès, Rosemary E. Kiernan, Laetitia K. Linares, Christine Chable-Bessia, Olga Plechakova, Céline Tréand, Stephane Emiliani, Jean-Marie Peloponese, Kuan-Teh Jeang, Olivier Coux, Martin Scheffner, and Monsef Benkirane
2003 - Nature Cell Biology
, 5, 754-761
Service porteur :
Laboratory of Molecular Virology
Tat acetyl-acceptor lysines are important for Human Immunodeficiency Virus type-1 replication
Brès, V., Rosemary Kiernan, Stéphane Emiliani and Monsef Benkirane
2002 - J. Biol. Chem. 277
, 22215-22221
Service porteur :
Laboratory of Molecular Virology
Differential acetylation of Tat coordinates its interaction with the co-activators cyclin T1 and PCAF.
Brès V. Tagami H. Péloponèse J.M. Loret E. Jeang K-T. Nakatani Y. Emiliani S. Benkirane M. and Kiernan ER.
2002 - EMBO.J.
, 21:6811-6819
Service porteur :
Laboratory of Molecular Virology
Interaction between cyclin T1 and SCFSKP2 targets CDK9 for ubiquitination and degradation by the proteasome.
Kiernan, R., Emiliani, S., Nakayama, K., Castro, A., Labbé, J.C., Lorca, T., Nakayama, K.-I., and Benkirane, M.
2001 - Mol. Cell. Biol.
, 21 (23), 7956-7970
Service porteur :
Laboratory of Molecular Virology
HIV-1 Tat transcriptional activity is regulated by acetylation.
Kiernan, R., Vanhulle, C., Schiltz, L., Adam, E., Xiao, H., Maudoux, F., Calomme, C., Burny, A., Nakatani, Y., Jeang, K.-T., Benkirane, M. and Van Lint, C.
1999 - EMBO J.
, 18, 6106-6118.
Service porteur :
Laboratory of Molecular Virology
