Gene regulation

All research teams

All organisms must regulate the expression of their genes to achieve the silencing of certain genes, and the activation of others during development and homeostasis. Disregulation of gene expression frequently has dire consequences, and can lead to pathologies such as cancer. The regulation of gene expression occurs at different levels, all of which depend on a multitude of factors. Chromatin is a primary regulator of gene expression. Physical compaction of the genome into chromatin controls accessability to the transcription machinery. Studies performed over recent years have revealed the enormous complexity involved in modifying chromatin to regulate gene expression.

KIERNAN Rosemary
Researcher

FRANCKHAUSER Celine
ITA

LE HARS Matthieu
IT

AKKAWI Charbel
Postdoc

PUBLICATIONS OF THE TEAM

Chromatin-associated MRN complex protects highly transcribing genes from genomic instability

Published the 21/05/2021 - Science Advances

Kader Salifou, Callum Burnard, Poornima Basavarajaiah, Marion Helsmoortel, Victor Mac, David Depierre, Giuseppa Grasso, Céline Franckhauser, Emmanuelle Beyne, Xavier Contreras, Jérôme Dejardin, Sylvie Rouquier, Olivier Cuvier, Rosemary Kiernan

PAF1 facilitates RNA polymerase II ubiquitination by the Elongin A complex through phosphorylation by CDK12

Published the 17/09/2020 - BioRxiv

Gabriel Sanchez, Jérôme Barbier, Céline Elie, Rosemary Kiernan, Sylvie Rouquier

NF90 modulates processing of a subset of human pri-miRNAs

Published the 19/05/2020 - Nucleic Acids Research

Grasso G, Higuchi T, Mac V, Barbier J, Helsmoortel M, Lorenzo C, Sanchez G, Bello M, Ritchie W, Sakamoto S, and Kiernan R.

[Nuclear RNA surveillance complexes control HIV-1 transcription].

Published the 19/02/2019 - Med Sci (Paris) , 35(2):113-115 30774074

Salifou K, Kiernan R, Contreras X

An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression.

Published the 23/03/2018 - Cell Res , 28(5):556-571 29563539

Barbier J, Chen X, Sanchez G, Cai M, Helsmoortel M, Higuchi T, Giraud P, Contreras X, Yuan G, Feng Z, Nait-Saidi R, Deas O, Bluy L, Judde JG, Rouquier S, Ritchie W, Sakamoto S, Xie D, Kiernan R

Nuclear RNA surveillance complexes silence HIV-1 transcription.

Published the 20/03/2018 - PLoS Pathog , 14(3):e1006950 29554134

Contreras X, Salifou K, Sanchez G, Helsmoortel M, Beyne E, Bluy L, Pelletier S, Rousset E, Rouquier S, Kiernan R

Identification of histone 3 variant 2 interacting factors

Published the 01/01/2014 - Nucleic Acids Res. , 42,6, 3542-3550 24393775

Latreille D, Bluy L, Benkirane M, Kiernan RE.

Premature termination of transcription by RNAP II: The beginning of the end.

Published the 01/01/2013 - Transcription , 4(2):72-6. 23714697

Contreras X, Benkirane M, Kiernan R.

Microprocessor, Setx, Xrn2 and Rrp6 Co-Operate to Induce Premature Termination of Transcription by RNAPII

Published the 01/01/2012 - CELL , 150, 1147-1157, http://dx.doi.org/10.1016/J.cell.2012.08.004 22980978

Wagschal, A., Rousset, E., Basavarajaiah, P., Contreras, X. , Harwig, A., Laurent-Chabalier, S. , Nakamura, M., Chen, X., Zhang, K., Meziane, O., Boyer, F., Parrinello, H., Berkhout, B., Terzian, C., Benkirane, M., Kiernan, R.

Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR

Published the 01/01/2012 - Retrovirology , 9(1):13 22316138

Nakamura M, Basavarajaiah P, Rousset E, Beraud C, Latreille D, Henaoui IS, Lassot I, Mari B, Kiernan R.

PUBLICATIONS COMMUNES

HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP

Sobhian, B., Laguette, N., Yatim, A., Nakamura, M., Levy, Y., Kiernan, R., Benkirane, M.
2010 - Molecular Cell , 38, 3, 439-451 20471949
Service porteur : Laboratory of Molecular Virology

Suv39H1 and HP1gamma are responsible for chromatin-mediated HIV-1 transcriptional silencing and post integration latency.

du Chéné, I., Basyuk, E., Lin,YL., Triboulet, R., Knezevich, A., Chable-Bessia, C., Mettling, C., Baillat, V., Reynes, J., Corbeau, P., Bertrand, E., Marcello, A., Emiliani, S., Kiernan, R., Benkirane, M.
2007 - EMBO.J. , 26(2):424-35. 17245432
Service porteur : Laboratory of Molecular Virology

Intrinsic ubiquitylation activity of PCAF controls the stability of the oncoprotein Hdm2

Linares, Laetitia, Rosemary Kiernan, Robinson Triboulet, Christine Chable-Bessia, Daniel Latreille, Olivier Cuvier, Matthieu Lacroix, Laurent Le Cam, Olivier Coux, and Monsef Benkirane.
2007 - Nature Cell Biology , 9(3):331-338 17293853
Service porteur : Laboratory of Molecular Virology

The Proteasome Regulates HIV-1 Transcription by Both Proteolytic and Non-Proteolytic Mechanisms

Lassot, L., Latreille, D., Rousset, E., Sourisseau, M., Linares, L.K., Chable-Bessia, C., Coux, O., Benkirane, M., Kiernan, R.
2007 - Molecular Cell , 25, 369-383 17289585
Service porteur : Laboratory of Molecular Virology

The HIV-1 Tat protein stimulates reverse transcription in vitro.

Apolloni, A., Meredith, LW., Suhrbier, A., Kiernan, R., Harrich, D
2007 - Curr. HIV Res. , 5 ,5, 473-483 17896967
Service porteur : Laboratory of Molecular Virology

Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter.

Treand C, du Chene I, Bres V, Kiernan R, Benarous R, Benkirane M, Emiliani S.
2006 - EMBO J. , 25, 1690-1699 16601680
Service porteur : Laboratory of Molecular Virology

A non proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter

Vanessa Brès, Rosemary E. Kiernan, Laetitia K. Linares, Christine Chable-Bessia, Olga Plechakova, Céline Tréand, Stephane Emiliani, Jean-Marie Peloponese, Kuan-Teh Jeang, Olivier Coux, Martin Scheffner, and Monsef Benkirane
2003 - Nature Cell Biology , 5, 754-761
Service porteur : Laboratory of Molecular Virology

Tat acetyl-acceptor lysines are important for Human Immunodeficiency Virus type-1 replication

Brès, V., Rosemary Kiernan, Stéphane Emiliani and Monsef Benkirane
2002 - J. Biol. Chem. 277 , 22215-22221
Service porteur : Laboratory of Molecular Virology

Differential acetylation of Tat coordinates its interaction with the co-activators cyclin T1 and PCAF.

Brès V. Tagami H. Péloponèse J.M. Loret E. Jeang K-T. Nakatani Y. Emiliani S. Benkirane M. and Kiernan ER.
2002 - EMBO.J. , 21:6811-6819
Service porteur : Laboratory of Molecular Virology

Interaction between cyclin T1 and SCFSKP2 targets CDK9 for ubiquitination and degradation by the proteasome.

Kiernan, R., Emiliani, S., Nakayama, K., Castro, A., Labbé, J.C., Lorca, T., Nakayama, K.-I., and Benkirane, M.
2001 - Mol. Cell. Biol. , 21 (23), 7956-7970
Service porteur : Laboratory of Molecular Virology

HIV-1 Tat transcriptional activity is regulated by acetylation.

Kiernan, R., Vanhulle, C., Schiltz, L., Adam, E., Xiao, H., Maudoux, F., Calomme, C., Burny, A., Nakatani, Y., Jeang, K.-T., Benkirane, M. and Van Lint, C.
1999 - EMBO J. , 18, 6106-6118.
Service porteur : Laboratory of Molecular Virology

HELSMOORTEL Marion
BioAster

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ELIE Céline
Bioaster

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SALIFOU Kader

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GIRAUD Pierre

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BEYNE Emmanuelle

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NOGARET Maguelone

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GRASSO Giuseppa

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MAC Victor

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TANCELIN Mathilde

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BERAUD Cyprien

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ASTIER William
Ecole Polytechnique Fédérale de Lausanne (EPFL), Suisse

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NAKAMURA Mirai

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CHEN Xin

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BASAVARAJAIAH Poornima

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COLENO-COSTES Anne
IGMM CNRS

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CRUNCHANT Eleonore

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PELLETIER Stéphane

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VERLEENE Fantine

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Topic: Role of NF90 in the miRNA pathway
Giuseppa Grasso from October 2017 to October 2021
Supervisor: Rosemary Kiernan