Gene regulation

All research teams

All organisms must regulate the expression of their genes to achieve the silencing of certain genes, and the activation of others during development and homeostasis. Disregulation of gene expression frequently has dire consequences, and can lead to pathologies such as cancer. The regulation of gene expression occurs at different levels, all of which depend on a multitude of factors. Chromatin is a primary regulator of gene expression. Physical compaction of the genome into chromatin controls accessability to the transcription machinery. Studies performed over recent years have revealed the enormous complexity involved in modifying chromatin to regulate gene expression.

Charbel AKKAWI
Researcher
NULL

Rosemary KIERNAN
Researcher
0434359935

Laurine LEMAIRE
Researcher
0434359935

Julie BOSSUYT
Ingénieur d'études en biologie moléculaire
ITA
0434359935

Marina SRBIC
ITA
NULL

Publications of the team

PAPγ associates with PAXT nuclear exosome to control the abundance of PROMPT ncRNAs

Published the 24/10/2023 - Nature Communications

Xavier Contreras, David Depierre, Charbel Akkawi, Marina Srbic, Marion Helsmoortel, Maguelone Nogaret, Matthieu LeHars, Kader Salifou, Alexandre Heurteau, Olivier Cuvier, Rosemary Kiernan

Chromatin-associated YTHDC1 coordinates heat-induced reprogramming of gene expression

Published the 13/12/2022 - Cell Reports , https://doi.org/10.1016/j.celrep.2022.111784

Kalina Timcheva, Solenne Dufour, Leila Touat-Todeschini, Callum Burnard, Marie-Christine Carpentier, Florent Chuffart, Rémy Merret, Marion Helsmoortel, Sabrina Ferré, Aude Grézy, Yohann Couté, Sophie Rousseau, Saadi Khochbin, Claire Vourc’h, Cécile Bousquet-Antonelli, Rosemary Kiernan, Daphné Seigneurin-Berny and André Verdel

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The Polyvalent Role of NF90 in RNA Biology

Published the 05/11/2022 - International Journal of Molecular Sciences

Giuseppa Grasso and Rosemary Kiernan

NF90 interacts with components of RISC and modulates association of Ago2 with mRNA.

Published the 02/09/2022 - BMC Biol , 20(1):194 36050755

Grasso G*, Akkawi C*, Franckhauser C, Nait-Saidi R, Bello M, Barbier J, Kiernan R

NF90 Interacts with Components of RISC and Modulates Association of Ago2 with mRNA

Published the 26/01/2022 - BioRxiv , doi: https://doi.org/10.1101/2021.09.23.461467

Giuseppa Grasso, Charbel Akkawi, Celine Franckhauser, Rima Nait-Saidi, Maxime Bello, Jérôme Barbier, Rosemary Kiernan

Chromatin-associated MRN complex protects highly transcribing genes from genomic instability

Published the 21/05/2021 - Science Advances

Kader Salifou, Callum Burnard, Poornima Basavarajaiah, Marion Helsmoortel, Victor Mac, David Depierre, Giuseppa Grasso, Céline Franckhauser, Emmanuelle Beyne, Xavier Contreras, Jérôme Dejardin, Sylvie Rouquier, Olivier Cuvier, Rosemary Kiernan

PAF1 facilitates RNA polymerase II ubiquitination by the Elongin A complex through phosphorylation by CDK12

Published the 17/09/2020 - BioRxiv

Gabriel Sanchez, Jérôme Barbier, Céline Elie, Rosemary Kiernan, Sylvie Rouquier

NF90 modulates processing of a subset of human pri-miRNAs

Published the 19/05/2020 - Nucleic Acids Research

Grasso G, Higuchi T, Mac V, Barbier J, Helsmoortel M, Lorenzo C, Sanchez G, Bello M, Ritchie W, Sakamoto S, Kiernan R.

[Nuclear RNA surveillance complexes control HIV-1 transcription].

Published the 19/02/2019 - Med Sci (Paris) , 35(2):113-115 30774074

Salifou K, Kiernan R, Contreras X

An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression.

Published the 23/03/2018 - Cell Res , 28(5):556-571 29563539

Barbier J, Chen X, Sanchez G, Cai M, Helsmoortel M, Higuchi T, Giraud P, Contreras X, Yuan G, Feng Z, Nait-Saidi R, Deas O, Bluy L, Judde JG, Rouquier S, Ritchie W, Sakamoto S, Xie D, Kiernan R

+ Machine learning and gene regulation

Nuclear RNA surveillance complexes silence HIV-1 transcription.

Published the 20/03/2018 - PLoS Pathog , 14(3):e1006950 29554134

Contreras X, Salifou K, Sanchez G, Helsmoortel M, Beyne E, Bluy L, Pelletier S, Rousset E, Rouquier S, Kiernan R

Identification of histone 3 variant 2 interacting factors

Published the 01/01/2014 - Nucleic Acids Res. , 42,6, 3542-3550 24393775

Latreille D, Bluy L, Benkirane M, Kiernan RE.

+ Laboratory of Molecular Virology

Premature termination of transcription by RNAP II: The beginning of the end.

Published the 01/01/2013 - Transcription , 4(2):72-6. 23714697

Contreras X, Benkirane M, Kiernan R.

+ Laboratory of Molecular Virology

Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR

Published the 01/01/2012 - Retrovirology , 9(1):13 22316138

Nakamura M, Basavarajaiah P, Rousset E, Beraud C, Latreille D, Henaoui IS, Lassot I, Mari B, Kiernan R.

Microprocessor, Setx, Xrn2 and Rrp6 Co-Operate to Induce Premature Termination of Transcription by RNAPII

Published the 01/01/2012 - CELL , 150, 1147-1157, http://dx.doi.org/10.1016/J.cell.2012.08.004 22980978

Wagschal, A., Rousset, E., Basavarajaiah, P., Contreras, X. , Harwig, A., Laurent-Chabalier, S. , Nakamura, M., Chen, X., Zhang, K., Meziane, O., Boyer, F., Parrinello, H., Berkhout, B., Terzian, C., Benkirane, M., Kiernan, R.

+ Laboratory of Molecular Virology

HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP

Published the 01/01/2010 - Molecular Cell , 38, 3, 439-451 20471949

Sobhian, B., Laguette, N., Yatim, A., Nakamura, M., Levy, Y., Kiernan, R., Benkirane, M.

+ Laboratory of Molecular Virology

The HIV-1 Tat protein stimulates reverse transcription in vitro.

Published the 01/01/2007 - Curr. HIV Res. , 5 ,5, 473-483 17896967

Apolloni, A., Meredith, LW., Suhrbier, A., Kiernan, R., Harrich, D

+ Laboratory of Molecular Virology

The Proteasome Regulates HIV-1 Transcription by Both Proteolytic and Non-Proteolytic Mechanisms

Published the 01/01/2007 - Molecular Cell , 25, 369-383 17289585

Lassot, L., Latreille, D., Rousset, E., Sourisseau, M., Linares, L.K., Chable-Bessia, C., Coux, O., Benkirane, M., Kiernan, R.

+ Laboratory of Molecular Virology

Intrinsic ubiquitylation activity of PCAF controls the stability of the oncoprotein Hdm2

Published the 01/01/2007 - Nature Cell Biology , 9(3):331-338 17293853

Linares, Laetitia, Rosemary Kiernan, Robinson Triboulet, Christine Chable-Bessia, Daniel Latreille, Olivier Cuvier, Matthieu Lacroix, Laurent Le Cam, Olivier Coux, and Monsef Benkirane.

+ Laboratory of Molecular Virology

Suv39H1 and HP1gamma are responsible for chromatin-mediated HIV-1 transcriptional silencing and post integration latency.

Published the 01/01/2007 - EMBO.J. , 26(2):424-35. 17245432

du Chéné, I., Basyuk, E., Lin,YL., Triboulet, R., Knezevich, A., Chable-Bessia, C., Mettling, C., Baillat, V., Reynes, J., Corbeau, P., Bertrand, E., Marcello, A., Emiliani, S., Kiernan, R., Benkirane, M.

+ Laboratory of Molecular Virology

Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter.

Published the 01/01/2006 - EMBO J. , 25, 1690-1699 16601680

Treand C, du Chene I, Bres V, Kiernan R, Benarous R, Benkirane M, Emiliani S.

+ Laboratory of Molecular Virology

A non proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter

Published the 01/01/2003 - Nature Cell Biology , 5, 754-761

Vanessa Brès, Rosemary E. Kiernan, Laetitia K. Linares, Christine Chable-Bessia, Olga Plechakova, Céline Tréand, Stephane Emiliani, Jean-Marie Peloponese, Kuan-Teh Jeang, Olivier Coux, Martin Scheffner, and Monsef Benkirane

+ Laboratory of Molecular Virology

Differential acetylation of Tat coordinates its interaction with the co-activators cyclin T1 and PCAF.

Published the 01/01/2002 - EMBO.J. , 21:6811-6819

Brès V. Tagami H. Péloponèse J.M. Loret E. Jeang K-T. Nakatani Y. Emiliani S. Benkirane M. and Kiernan ER.

+ Laboratory of Molecular Virology

Tat acetyl-acceptor lysines are important for Human Immunodeficiency Virus type-1 replication

Published the 01/01/2002 - J. Biol. Chem. 277 , 22215-22221

Brès, V., Rosemary Kiernan, Stéphane Emiliani and Monsef Benkirane

+ Laboratory of Molecular Virology

Interaction between cyclin T1 and SCFSKP2 targets CDK9 for ubiquitination and degradation by the proteasome.

Published the 01/01/2001 - Mol. Cell. Biol. , 21 (23), 7956-7970

Kiernan, R., Emiliani, S., Nakayama, K., Castro, A., Labbé, J.C., Lorca, T., Nakayama, K.-I., and Benkirane, M.

+ Laboratory of Molecular Virology

HIV-1 Tat transcriptional activity is regulated by acetylation.

Published the 01/01/1999 - EMBO J. , 18, 6106-6118.

Kiernan, R., Vanhulle, C., Schiltz, L., Adam, E., Xiao, H., Maudoux, F., Calomme, C., Burny, A., Nakatani, Y., Jeang, K.-T., Benkirane, M. and Van Lint, C.

+ Laboratory of Molecular Virology

Thèses et hdr

Role of NF90 in the miRNA pathway 08/11/2021

Defended by Giuseppa Grasso on 08/11/2021 under the supervision of Rosemary Kiernan

Gene regulation

Publications of the team

PAPγ associates with PAXT nuclear exosome to control the abundance of PROMPT ncRNAs

Chromatin-associated YTHDC1 coordinates heat-induced reprogramming of gene expression

The Polyvalent Role of NF90 in RNA Biology

NF90 interacts with components of RISC and modulates association of Ago2 with mRNA.

NF90 Interacts with Components of RISC and Modulates Association of Ago2 with mRNA

Chromatin-associated MRN complex protects highly transcribing genes from genomic instability

PAF1 facilitates RNA polymerase II ubiquitination by the Elongin A complex through phosphorylation by CDK12

NF90 modulates processing of a subset of human pri-miRNAs

[Nuclear RNA surveillance complexes control HIV-1 transcription].

An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression.

Nuclear RNA surveillance complexes silence HIV-1 transcription.

Identification of histone 3 variant 2 interacting factors

Premature termination of transcription by RNAP II: The beginning of the end.

Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR

Microprocessor, Setx, Xrn2 and Rrp6 Co-Operate to Induce Premature Termination of Transcription by RNAPII

HIV-1 Tat assembles a multifunctional transcription elongation complex and stably associates with the 7SK snRNP

The HIV-1 Tat protein stimulates reverse transcription in vitro.

The Proteasome Regulates HIV-1 Transcription by Both Proteolytic and Non-Proteolytic Mechanisms

Intrinsic ubiquitylation activity of PCAF controls the stability of the oncoprotein Hdm2

Suv39H1 and HP1gamma are responsible for chromatin-mediated HIV-1 transcriptional silencing and post integration latency.

Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter.

A non proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter

Differential acetylation of Tat coordinates its interaction with the co-activators cyclin T1 and PCAF.

Tat acetyl-acceptor lysines are important for Human Immunodeficiency Virus type-1 replication

Interaction between cyclin T1 and SCFSKP2 targets CDK9 for ubiquitination and degradation by the proteasome.

HIV-1 Tat transcriptional activity is regulated by acetylation.

Thèses et hdr

Role of NF90 in the miRNA pathway 08/11/2021

Head : Rosemary KIERNAN

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