Biology of Repetitive Sequences

Genome dynamics

Chromatin can be viewed as a highly complex mixture of proteins and nucleic acids that orchestrate DNA-based processes in the eukaryotic genome. Most of the mammalian genome is assembled into heterochromatin, a ‘closed’ structure imposed by several enzymatic activities. Such activities act on histones and the DNA itself to impinge on transcription, replication or repair.
Most of the heterochromatic fraction of the genome can be found at critical loci. These include telomeres, repetitive sequences around centromeres and a portion (about half) of the gene units encoding ribosomal RNAs. Defects in the regulation of these loci have therefore disastrous consequences on cell identity and can lead to developmental problems, cancer, premature aging or immune deficiencies. How precisely heterochromatic enzymes affect the composition of target loci has remained elusive and research in our laboratory primarily focuses on this question.
To understand how heterochromatin acts at the molecular level, we are looking at the effect of abrogating important heterochromatic activities, such as histone and/or DNA methyl-transferases, on the overall composition of key heterochromatic loci (telomeres, pericentromeres and rDNA).

Jerome DEJARDIN
Researcher

0434359945
Mathieu Tardat
Mathieu TARDAT
Researcher

0434359945
Gabrielle Pozo
Gabrielle POZO
IT

0434359945
Sandrine Sauzet
Sandrine SAUZET
IT

0434359945
Constance Kleijwegt
Constance KLEIJWEGT
Postdoc

0434359945
Joey Dufourd
Joey DUFOURD
PhD Student

0434359945
Elise Gardes
Elise GARDES
Doctorante
PhD Student

0434359945
Thi Hai Yen Huynh
Thi Hai Yen HUYNH
Phd
PhD Student

0434359945
Solene Le Bolloch
Solene LE BOLLOCH
Stagiaire

0434359959

Publications of the team

Purification and enrichment of specific chromatin loci.

Gauchier M, van Mierlo G, Vermeulen M, Déjardin J

Telomeric Chromatin and TERRA.

Barral A, Déjardin J

Locus-specific chromatin isolation.

Vermeulen M, Déjardin J

Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins.

Traynor S, Møllegaard NE, Jørgensen MG, Brückmann NH, Pedersen CB, Terp MG, Johansen S, Dejardin J, Ditzel HJ, Gjerstorff MF

SMCHD1 is involved in de novo methylation of the DUX4-encoding D4Z4 macrosatellite.

Dion C, Roche S, Laberthonnière C, Broucqsault N, Mariot V, Xue S, Gurzau AD, Nowak A, Gordon CT, Gaillard MC, El-Yazidi C, Thomas M, Schlupp-Robaglia A, Missirian C, Malan V, Ratbi L, Sefiani A, Wollnik B, Binetruy B, Salort Campana E, Attarian S, Bernard R, Nguyen K, Amiel J, Dumonceaux J, Murphy JM, Déjardin J, Blewitt ME, Reversade B, Robin JD, Magdinier F

Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency.

van Mierlo G, Dirks RAM, De Clerck L, Brinkman AB, Huth M, Kloet SL, Saksouk N, Kroeze LI, Willems S, Farlik M, Bock C, Jansen JH, Deforce D, Vermeulen M, Déjardin J, Dhaenens M, Marks H

Telomere chromatin establishment and its maintenance during mammalian development.

Tardat M, Déjardin J

Histone H4K20 tri-methylation at late-firing origins ensures timely heterochromatin replication

Brustel J, Kirstein N, Izard F, Grimaud C, Prorok P, Cayrou C, Schotta G, Abdelsamie AF, Déjardin J, Méchali M, Baldacci G, Sardet C, Cadoret JC, Schepers A, Julien E.

+ Replication and Genome Dynamics

Proteome Characterization of a Chromatin Locus Using the Proteomics of Isolated Chromatin Segments Approach

Kan SL, Saksouk N, Déjardin J

The molecular basis of the organization of repetitive DNA-containing constitutive heterochromatin in mammals

Nishibuchi G, Déjardin J.

The cell proliferation antigen Ki-67 organises heterochromatin

Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Llères D, Gerbe F, Prieto S, Krasinska L, David A, Eguren M, Birling MC, Urbach S, Hem S, Déjardin J, Malumbres M, Jay P, Dulic V, Lafontaine DL, Feil RP, Fisher D

Switching between Epigenetic States at Pericentromeric Heterochromatin

Dejardin, J.

End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter

Ide, S., Dejardin, J.

Constitutive heterochromatin formation and transcription in mammals

Saksouk N, Simboeck E, Déjardin J.

Nuclear-Receptor-Mediated Telomere Insertion Leads to Genome Instability in ALT Cancer

Marzec, P., Armenise, C., Perot, G., Roumelioti, F.M., Basyuk, E., Gagos, S., Chibon, F., Dejardin, J.

Redundant Mechanisms to Form Silent Chromatin at Pericentromeric Regions Rely on BEND3 and DNA Methylation

Saksouk N, Barth TK, Ziegler-Birling C, Olova N, Nowak A, Rey E, Mateos-Langerak J, Urbach S, Reik W, Torres-Padilla ME, Imhof A, Déjardin J.

How chromatin prevents genomic rearrangements: Locus colocalization induced by transcription factor binding.

Dejardin, J.

KAN Sophie
KAN Sophie
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GAUCHIER Mathilde
GAUCHIER Mathilde
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BARRAL Amandine
BARRAL Amandine
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PLINET Mathilde
PLINET Mathilde
Dendris
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GUINTINI Laëtitia
GUINTINI Laëtitia
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GARCIA MOLINERO Varinia
GARCIA MOLINERO Varinia
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ZITOUNI Sihem
ZITOUNI Sihem
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DUCROT Lucas
DUCROT Lucas
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HAMADE Bariaa
HAMADE Bariaa
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REY Elodie
REY Elodie
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NOWAK Agnieszka
NOWAK Agnieszka
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ARMENISE Claudia
ARMENISE Claudia
Fasteris, Life Science Genesupport Switzerland
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SIMBOECK Elisabeth
SIMBOECK Elisabeth
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BOUHAYANE Assia
BOUHAYANE Assia
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