Biology of Repetitive Sequences

Genome dynamics

Chromatin can be viewed as a highly complex mixture of proteins and nucleic acids that orchestrate DNA-based processes in the eukaryotic genome. Most of the mammalian genome is assembled into heterochromatin, a ‘closed’ structure imposed by several enzymatic activities. Such activities act on histones and the DNA itself to impinge on transcription, replication or repair.
Most of the heterochromatic fraction of the genome can be found at critical loci. These include telomeres, repetitive sequences around centromeres and a portion (about half) of the gene units encoding ribosomal RNAs. Defects in the regulation of these loci have therefore disastrous consequences on cell identity and can lead to developmental problems, cancer, premature aging or immune deficiencies. How precisely heterochromatic enzymes affect the composition of target loci has remained elusive and research in our laboratory primarily focuses on this question.
To understand how heterochromatin acts at the molecular level, we are looking at the effect of abrogating important heterochromatic activities, such as histone and/or DNA methyl-transferases, on the overall composition of key heterochromatic loci (telomeres, pericentromeres and rDNA).

Jerome DEJARDIN
Researcher

0434359945
Constance Kleijwegt
Constance KLEIJWEGT
Researcher

0434359945
Mathieu TARDAT
Researcher

0434359945
Joey Dufourd
Joey DUFOURD
PhD Student

0434359945
Elise Gardes
Elise GARDES
Doctorante
PhD Student

0434359945
Thi Hai Yen Huynh
Thi Hai Yen HUYNH
Phd
PhD Student

0434359945
Sandrine Sauzet
Sandrine SAUZET
ITA

0434359945
Mathieu Missland
Mathieu MISSLAND
Stagiaire

0411759728

Publications of the team

Purification and enrichment of specific chromatin loci.

Gauchier M, van Mierlo G, Vermeulen M, Déjardin J

Telomeric Chromatin and TERRA.

Barral A, Déjardin J

Locus-specific chromatin isolation.

Vermeulen M, Déjardin J

Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins.

Traynor S, Møllegaard NE, Jørgensen MG, Brückmann NH, Pedersen CB, Terp MG, Johansen S, Dejardin J, Ditzel HJ, Gjerstorff MF

SMCHD1 is involved in de novo methylation of the DUX4-encoding D4Z4 macrosatellite.

Dion C, Roche S, Laberthonnière C, Broucqsault N, Mariot V, Xue S, Gurzau AD, Nowak A, Gordon CT, Gaillard MC, El-Yazidi C, Thomas M, Schlupp-Robaglia A, Missirian C, Malan V, Ratbi L, Sefiani A, Wollnik B, Binetruy B, Salort Campana E, Attarian S, Bernard R, Nguyen K, Amiel J, Dumonceaux J, Murphy JM, Déjardin J, Blewitt ME, Reversade B, Robin JD, Magdinier F

Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency.

van Mierlo G, Dirks RAM, De Clerck L, Brinkman AB, Huth M, Kloet SL, Saksouk N, Kroeze LI, Willems S, Farlik M, Bock C, Jansen JH, Deforce D, Vermeulen M, Déjardin J, Dhaenens M, Marks H

Telomere chromatin establishment and its maintenance during mammalian development.

Tardat M, Déjardin J

Histone H4K20 tri-methylation at late-firing origins ensures timely heterochromatin replication

Brustel J, Kirstein N, Izard F, Grimaud C, Prorok P, Cayrou C, Schotta G, Abdelsamie AF, Déjardin J, Méchali M, Baldacci G, Sardet C, Cadoret JC, Schepers A, Julien E.

+ Replication and Genome Dynamics

Proteome Characterization of a Chromatin Locus Using the Proteomics of Isolated Chromatin Segments Approach

Kan SL, Saksouk N, Déjardin J

The molecular basis of the organization of repetitive DNA-containing constitutive heterochromatin in mammals

Nishibuchi G, Déjardin J.

The cell proliferation antigen Ki-67 organises heterochromatin

Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Llères D, Gerbe F, Prieto S, Krasinska L, David A, Eguren M, Birling MC, Urbach S, Hem S, Déjardin J, Malumbres M, Jay P, Dulic V, Lafontaine DL, Feil RP, Fisher D

Switching between Epigenetic States at Pericentromeric Heterochromatin

Dejardin, J.

End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter

Ide, S., Dejardin, J.

Constitutive heterochromatin formation and transcription in mammals

Saksouk N, Simboeck E, Déjardin J.

Nuclear-Receptor-Mediated Telomere Insertion Leads to Genome Instability in ALT Cancer

Marzec, P., Armenise, C., Perot, G., Roumelioti, F.M., Basyuk, E., Gagos, S., Chibon, F., Dejardin, J.

Redundant Mechanisms to Form Silent Chromatin at Pericentromeric Regions Rely on BEND3 and DNA Methylation

Saksouk N, Barth TK, Ziegler-Birling C, Olova N, Nowak A, Rey E, Mateos-Langerak J, Urbach S, Reik W, Torres-Padilla ME, Imhof A, Déjardin J.

How chromatin prevents genomic rearrangements: Locus colocalization induced by transcription factor binding.

Dejardin, J.

Thèses et hdr

SETDB1 and NSDs-dependent dual heterochromatin H3K9me3/H3K36me3 ensures gene expression by keeping enhancers inactive 07/07/2021

GARCIA MOLINERO Varinia
GARCIA MOLINERO Varinia
Chercheur
GAUCHIER Mathilde
GAUCHIER Mathilde
Chercheur
GUINTINI Laëtitia
GUINTINI Laëtitia
Chercheur
IDE Satoru
IDE Satoru
Chercheur
KLEIJWEGT Constance
KLEIJWEGT Constance
Chercheur
NISHIBUCHI Gohei
NISHIBUCHI Gohei
Chercheur
NOWAK Agnieszka
NOWAK Agnieszka
Chercheur
SIMBOECK Elisabeth
SIMBOECK Elisabeth
Chercheur
ZITOUNI Sihem
ZITOUNI Sihem
Chercheur
BARRAL Amandine
BARRAL Amandine
Doctorant
ARMENISE Claudia
ARMENISE Claudia
ITA
BOUHAYANE Assia
BOUHAYANE Assia
ITA
DUCROT Lucas
DUCROT Lucas
ITA
HAMADE Bariaa
HAMADE Bariaa
ITA
KAN Sophie
KAN Sophie
ITA
MARZEC Paulina
MARZEC Paulina
ITA
PLINET Mathilde
PLINET Mathilde
ITA
POZO Gabrielle
POZO Gabrielle
ITA
REY Elodie
REY Elodie
ITA