Project : Roles of Trim28, a partner of Sox9 in sexual differentiation

Using a genetic approach in mice, we are currently investigating the role of Trim28 in embryonic and adult supporting cell lines.

Since complete knockout is lethal in early embryonic stages, we used a conditional "floxed" allele associated with Sf1-Cre or Wt1-CreERT2 to invalidate Trim28 in Granulosa and Sertoli cells at different developmental stages and after birth.

Transcriptomic, ChIP-seq and in vitro approaches are also used to decipher the role of Trim28 in ovarian identity maintenance and sexual differentiation.