Orateur:
Bennett Van Houten (UPMC-Hillman Cancer Center, PA, USA)
Genopolys, Room Rotonde
A new single molecule approach to study DNA repair protein dynamics: seeing is believing
This seminar will present a new rapid and robust method for single molecule analysis of DNA binding proteins from nuclear extracts (SMADNE) of human cells expressing a fluorescently tagged protein of interest (Schaich et al., Nucleic Acids Res. 2023 Apr 24;51(7):e39). SMADNE when combined with the LUMICKS C-trap provides unprecedented observations of DNA repair protein dynamics on damaged DNA. Over the last 18 months our group has been able to analyze the dwell times of 30 proteins or protein variants...
Orateur:
Patrick Lomonte (Univ Lyon, Université Claude Bernard Lyon 1)
IGH seminars room
Epigenetic control of herpes simplex virus 1 latency
The establishment of latent herpes simplex virus 1 (HSV-1) infection involves an intricate interplay of cellular and molecular processes. On the epigenetic front, this entails viral chromatinization and the interaction between latent episomal viral genomes and the cell's nucleus. Promyelocytic leukemia nuclear bodies (PML NBs) are nuclear membrane-less organelles responding to interferon stimuli, and playing a major role in the transcriptional regulation of HSV-1 latent genomes through an intrinsic...
Mammalian genomes contain hundreds of lamina-associated domains (LADs), which are large, often megabase-sized heterochromatin domains that are anchored to the nuclear lamina (NL). Even though LADs play a key role in the spatial organization of the genome and potently repress gene activity, it is not yet understood how their interactions with the NL are encoded in their DNA. Here, we investigated the principles that govern LAD-NL interactions by taking a "LAD scrambling" approach. This approach relies...
Proximity Copy Paste (PCP) : A new method to map the 3D organization of chromatin
Proximity Copy Paste (PCP): A new method to map the 3D organization of chromatin
Understanding the principles of three-dimensional genome organization is essential to comprehend genome maintenance, expression, and duplication. Current Hi-C based methods use fragmentation of crosslinked chromatin followed by proximity ligation to detect DNA molecules whose extremities can be ligated together. While powerful, these approaches are limited because only ligatable, pairwise interactions are mapped,...
Orateur:
Shan Gao (Ocean University of China)
Genopolys Amphitheater
de novo methylation of N6-methyladenine (6mA) showcased in the unicellular eukaryote Tetrahymena
DNA N6-adenine methylation (6mA) is rediscovered as an important epigenetic mark in eukaryotes. It however remains unclear what the de novo methyltransferases (MTases) are and how they fulfill their biological functions. The binucleated Tetrahymena thermophila happens to possess a unique time window for the study of de novo methylation, when the 6mA-free zygotic nucleus develops into 6mA-positive new macronucleus (MAC). In this study, we found that the MT-A70 family members, AMT2 and AMT5, are not...
