de novo methylation of N6-methyladenine (6mA) showcased in the unicellular eukaryote Tetrahymena
Epigenetic Chromatin Regulation
DNA N6-adenine methylation (6mA) is rediscovered as an important epigenetic mark in eukaryotes. It however remains unclear what the de novo methyltransferases (MTases) are and how they fulfill their biological functions. The binucleated Tetrahymena thermophila happens to possess a unique time window for the study of de novo methylation, when the 6mA-free zygotic nucleus develops into 6mA-positive new macronucleus (MAC). In this study, we found that the MT-A70 family members, AMT2 and AMT5, are not required for the maintenance 6mA in the vegetative MAC but for de novo 6mA in the new MAC. As a consequence loss of AMT2 and/or AMT5 reduces the fitness of sexual progeny.
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A Tribute to Angelos Constantinou
du
10/03/2025
jusqu'au 13/03/2025
Village Club de Carry-Le-Rouet, France
