Tartier et al. review the challenges and strategies for purifying biomolecular condensates, emphasizing that their dynamic and heterogeneous nature requires careful cell lysis and rigorous validation to preserve integrity, and also highlighting the paradox that condensates appear more robust in vivo than when reconstituted in vitro.

The purification of biomolecular condensates: Bottlenecks and strategies
Tartier et al. Journal of Cell Biology 2025
https://doi.org/10.1083/jcb.202504081

Edouard Bertrand - Cell biology of RNA